Human Exodus 2 ELISA Kit (CCL21) (ab193759)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.8 pg/ml
- Range: 9.4 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Exodus 2 ELISA Kit (CCL21) -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 3 3.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 3 5.5% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
3.8 pg/ml -
検出範囲
9.4 pg/ml - 600 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 104 102% - 106% Cell culture media 99 94% - 105% Hep Plasma 102 99% - 106% Cit plasma 99 93% - 106% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Exodus 2 ELISA Kit (CCL21) (ab193759) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Exodus 2 (CCL21) protein in cell culture supernatant, cit plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Exodus 2 (CCL21) with 3.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Exodus 2 (CCL 21) is a small cytokine in the CC chemokine family, and is involved in inhibition of hemopoiesis and stimulation of chemotaxis. It interacts with atypical chemokine receptor ACKR4 and recruits beta-arrestin to ACKR4.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 1 x 96 tests 10X Human Exodus 2 Capture Antibody 1 x 600µl 1 x 600µl 10X Human Exodus 2 Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml Antibody Diluent CPI2 1 x 6ml 1 x 6ml Human Exodus 2 Lyophilized Recombinant Protein 2 vials 2 vials Plate Seals 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit Stop Solution 1 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 1 x 12ml -
研究分野
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機能
Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. -
組織特異性
Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- 6Ckine
- Beta chemokine exodus 2
- Beta-chemokine exodus-2
see all -
参照データベース
- Entrez Gene: 6366 Human
- Omim: 602737 Human
- SwissProt: O00585 Human
- Unigene: 57907 Human
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD, n = 3) are graphed
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Mean Human Exodus 2 values fall within expected normal reference ranges (WHO).
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Mean Human Exodus 2 is 479 pg/mL and values fall within expected normal reference ranges (WHO).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab193759 は 3 報の論文で使用されています。
- Chen B et al. Long non-coding RNA H19 protects against intracerebral hemorrhage injuries via regulating microRNA-106b-5p/acyl-CoA synthetase long chain family member 4 axis. Bioengineered 12:4004-4015 (2021). PubMed: 34288826
- Suh KJ et al. EGFR or HER2 inhibition modulates the tumor microenvironment by suppression of PD-L1 and cytokines release. Oncotarget 8:63901-63910 (2017). ELISA . PubMed: 28969039
- Yamashita M et al. Lymphangiogenic factors are associated with the severity of hypersensitivity pneumonitis. BMJ Open Respir Res 2:e000085 (2015). ELISA ; Human . PubMed: 26448865