Human eIF4E Matched 抗体 Pair Kit (ab220125)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 125 pg/ml - 8000 pg/ml
製品の概要
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製品名
Human eIF4E Matched Antibody Pair Kit
eIF4E キット 製品一覧 -
検出方法
Colorimetric -
アッセイタイプ
ELISA set -
検出感度
27.8 pg/ml -
検出範囲
125 pg/ml - 8000 pg/ml -
種交差性
交差種: Human -
製品の概要
Human eIF4E Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human eIF4E.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214564. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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アプリケーション
適用あり: ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 5 x 96 tests Human eIF4E Capture Antibody 1 x 50µg Human eIF4E Detector Antibody 1 x 12.5µg Human eIF4E Lyophilized Protein 1 vial -
研究分野
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機能
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. -
配列類似性
Belongs to the eukaryotic initiation factor 4E family. -
翻訳後修飾
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. - Information by UniProt
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別名
- AUTS19
- CBP
- eIF 4E
see all -
参照データベース
- Entrez Gene: 1977 Human
- Omim: 133440 Human
- SwissProt: P06730 Human
- Unigene: 249718 Human
関連製品
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Alternative Versions
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Related Products
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SimpleStep ELISA kits
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab220125の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
画像
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Standard calibration curve. Background subtracted values are graphed.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab220125 は論文での使用が確認できていません。