Human DPT ELISA Kit (TRAMP) (ab272206)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.94 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture media, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human DPT ELISA Kit (TRAMP)
DPT/TRAMP キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 3.2% -
サンプルの種類
Serum, Cell culture media, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2.94 pg/ml -
検出範囲
18.75 pg/ml - 1200 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 107 % - % Cell culture media 100 % - % Cit plasma 102 % - % -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human DPT ELISA kit (ab272206) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DPT protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DPT with 2.94 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
DPT (TRAMP or Dermatopontin) is a matrix protein found in the intracellular junction of the extracellular matrix. DPT is increased in myocardial infarction and decreased in fibrosis and leiomyoma. DPT is a biomarker for giant-cell tumor of the bone and oral squamous cell carcinoma.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human DPT (TRAMP) Capture Antibody 1 x 600µl 10X Human DPT (TRAMP) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human DPT (TRAMP) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation. -
組織特異性
Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts. -
配列類似性
Belongs to the dermatopontin family. -
翻訳後修飾
Sulfated on tyrosine residue(s). -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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別名
- DERM_HUMAN
- Dermatopontin
- Dpt
see all -
参照データベース
- Entrez Gene: 1805 Human
- Omim: 125597 Human
- SwissProt: Q07507 Human
- Unigene: 80552 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The DPT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of DPT were measured in duplicates, interpolated from the DPT standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 100 and plasma (citrate) 1: 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DPT concentration was determined to be 53.79 ng/mL in serum and 63.04 ng/mL in plasma (citrate).
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272206 は論文での使用が確認できていません。