Human DKK3 ELISA Kit (Dickkopf-3) (ab100502)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human DKK3 ELISA Kit (Dickkopf-3) -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 3 pg/ml -
検出範囲
2.74 pg/ml - 2000 pg/ml -
添加回収試験
> 85 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 96.23 85% - 105% Serum 86.69 77% - 96% Plasma 89.24 80% - 98% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s DKK3 (Dickkopf-3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK3 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human DKK3 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimisation may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human DKK3 2 vials DKK3 Microplate (12 x 8 well strips) 1 unit Recombinant Human DKK3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性
DKK3, like DKK1, DKK2, and DKK4, possesses an N terminal signal peptide and 2 conserved cysteine rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin type proteases. -
細胞内局在
Secreted -
別名
- AW061014
- C87148
- Dickkopf (Xenopus laevis) homolog 3
see all -
参照データベース
- Entrez Gene: 27122 Human
- Omim: 605416 Human
- SwissProt: Q9UBP4 Human
画像
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Typical Standard Curve
Representative Standard Curve using ab100502.
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Typical Standard CurveRepresentative Standard Curve using ab100502.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab100502 は 2 報の論文で使用されています。
- Qaisar R et al. Circulating Biomarkers of Accelerated Sarcopenia in Respiratory Diseases. Biology (Basel) 9:N/A (2020). PubMed: 33023021
- Shin JM et al. The expression pattern and functional role of REIC/Dkk-3 in the development of cutaneous squamous cell carcinoma. J Dermatol Sci 84:88-96 (2016). PubMed: 27354306