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Human Anti-Cytomegalovirus IgG ELISA Kit (CMV) (ab108639)

Reviews (1) Submit a question References (2)
Typical Standard Curve

    Key features and details

    • Sample type: Serum
    • Detection method: Colorimetric
    • Assay type: Indirect
    • Reacts with: Human

    製品の概要

    • 製品名

      Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)

    • 検出方法

      Colorimetric

    • サンプルの種類

      Serum

    • アッセイタイプ

      Indirect

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s anti-Cytomegalovirus (CMV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Cytomegalovirus in Human serum.

      A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Cytomegalovirus antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgG sample captured in plate.

    • 試験プラットフォーム

      Microplate

    製品の特性

    画像

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab108639

    参考文献 (2)

    ab108639 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108639 は 2 報の論文で使用されています。

    • Lustig A  et al. Telomere Shortening, Inflammatory Cytokines, and Anti-Cytomegalovirus Antibody Follow Distinct Age-Associated Trajectories in Humans. Front Immunol 8:1027 (2017). PubMed: 28970831
    • Lin Y  et al. Changes in blood lymphocyte numbers with age in vivo and their association with the levels of cytokines/cytokine receptors. Immun Ageing 13:24 (2016). ELISA ; Human . PubMed: 27547234

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Plasma does not impact ability of Cytomegalovirus IgG Human ELISA Kit (Cat #ab108639) to determine CMV IgG positivity

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image
    Use of plasma does not impact ability of Cytomegalovirus IgG Human ELISA Kit (Cat #ab108639) to determine CMV IgG positivity
    n = 10 (matched serum and plasma samples i.e. from same blood draw)

    Basic assessment of whether plasma affects the performance of the assay performed by spiking the Cytomegalovirus IgG Standards provided with the Kit into matched serum and plasma samples
    Average recovery across standards of 87 % for serum and 84 % for plasma
    Average recovery for each standard in 80% - 120% acceptance range for immunoassays
    Note:
    CMV IgG Index values used to assess recovery
    Assessment of "Recovery" as described in A practical guide to immunoassay method validation. Front. Neurol. 6:179.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    投稿 Aug 19 2020

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