Human Cytoglobin ELISA (ab277460)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.287 ng/ml - 70 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human Cytoglobin ELISA -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall < 12% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.3 ng/ml -
検出範囲
0.287 ng/ml - 70 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 90.59 82% - 101% Plasma 87.98 84% - 94% Cell culture media 74.86 69% - 87% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human Cytoglobin ELISA Kit (ab277460) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cytoglobin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Cytoglobin coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Cytoglobin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Cytoglobin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Cytoglobin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human Cytoglobin Antibody 2 vials Anti-Human Cytoglobin coated Microplate 1 unit Human Cytoglobin standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
機能
May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer. -
組織特異性
Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine. -
配列類似性
Belongs to the globin family. -
細胞内局在
Cytoplasm. - Information by UniProt
-
別名
- Cygb
- CYGB_HUMAN
- Cytoglobin
see all -
参照データベース
- Entrez Gene: 114757 Human
- Omim: 608759 Human
- SwissProt: Q8WWM9 Human
- Unigene: 95120 Human
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab277460 は論文での使用が確認できていません。