Human Complement C7 ELISA Kit (ab125964)
Key features and details
- Sensitivity: 0.17 ng/ml
- Range: 0.3125 ng/ml - 20 ng/ml
- Sample type: Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Complement C7 ELISA Kit
C7 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 9.9% -
サンプルの種類
Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.17 ng/ml -
検出範囲
0.313 ng/ml - 20 ng/ml -
添加回収試験
97 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Complement C7 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of C7 in Human plasma, serum, urine, milk, saliva, and CSF samples.
A Complement C7 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C7 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C7 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C7 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Complement C7 Microplate (12 x 8 well strips) 1 unit Complement C7 Standard (Lyophilized) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. -
関連疾患
Defects in C7 are a cause of complement component 7 deficiency (C7D) [MIM:610102]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. -
配列類似性
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 2 Sushi (CCP/SCR) domains.
Contains 2 TSP type-1 domains. -
翻訳後修飾
C7 has 28 disulfide bridges. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C7
- CO7_HUMAN
- complement component 7
- Complement component C7
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参照データベース
- Entrez Gene: 730 Human
- Omim: 217070 Human
- SwissProt: P10643 Human
- Unigene: 78065 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab125964 は 2 報の論文で使用されています。
- Abdel-Tawab MS et al. Evaluation of Serum and Gene Expression of Galectin-4, Interleukin-27, and Complement-7 in Hepatitis C Virus-Infected Egyptian Patients. Biomed Res Int 2020:8879758 (2020). PubMed: 33381596
- Liang Y et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912