Human Complement C5 ELISA Kit (ab125963)

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製品の概要

  • 製品名

    Human Complement C5 ELISA Kit
    C5 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Overall 4.1%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Overall 9.7%

  • サンプルの種類

    Cell culture supernatant, Saliva, Milk, Serum, Plasma, Cerebral Spinal Fluid

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    0.1 ng/ml

  • 検出範囲

    0.313 ng/ml - 20 ng/ml

  • 添加回収試験

    101 %

  • 全工程の試験時間

    4h 00m

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human Complement C5 ELISA kit (Enzyme-Linked Immunosorbent Assay) is designed for the quantitative measurement of C5 in human plasma, serum, saliva, milk, CSF and cell culture supernatants.


    A Complement C5 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C5 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C5 captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Complement C5 Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Complement C5 Microplate (12 x 8 well strips) 1 unit
    Complement C5 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml

  • 研究分野

  • 機能

    Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).

  • 関連疾患

    Defects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

  • 配列類似性

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 別名

    • Anaphylatoxin C5a analog
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
    • C5
    • C5a
    • C5a anaphylatoxin
    • C5b
    • CO5_HUMAN
    • Complement C5
    • Complement C5 alpha'' chain
    • Complement component C5
    • CPAMD4
    • prepro-C5
    see all

  • 参照データベース

    画像

    • Sandwich ELISA - Complement C5 Human ELISA Kit (ab125963)
      Sandwich ELISA - Complement C5 Human ELISA Kit (ab125963)
      Complement 5 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
    • Typical Stand Curve
      Typical Stand Curve

      Representative Standard Curve using ab125963

    参考文献

    ab125963 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab125963 は 5 報の論文で使用されています。

    • Meng X  et al. DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma. EBioMedicine 41:185-199 (2019). PubMed: 30773478
    • Kusner LL  et al. Investigational RNAi Therapeutic Targeting C5 Is Efficacious in Pre-clinical Models of Myasthenia Gravis. Mol Ther Methods Clin Dev 13:484-492 (2019). PubMed: 31193726
    • Csosz É  et al. Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population. FEBS Open Bio 8:556-569 (2018). PubMed: 29632809
    • Guerra AD  et al. Minocycline modulates NF?B phosphorylation and enhances antimicrobial activity against Staphylococcus aureus in mesenchymal stromal/stem cells. Stem Cell Res Ther 8:171 (2017). ELISA ; Human . PubMed: 28732530
    • Owsley C  et al. Associations between abnormal rod-mediated dark adaptation and health and functioning in older adults with normal macular health. Invest Ophthalmol Vis Sci 55:4776-89 (2014). ELISA ; Human . PubMed: 24854857

    レビューと Q&A

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    Question

    I have a question in reference to your C5 kit (ab125963). Specifically, I am looking for an assay that detects only unactivated C5, not C5a or C5a-des-Arg. I was not clear from your product information if your ELISA measures both C5 and C5a or just the unactivated form. Can you clarify this for me?

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    Answer

    Thank you for contacting Abcam regarding ab125963.


    I have confirmed that this kit will react withboth C5 and C5a.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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    Question

    Is the protein used as standard from a native source or rather recombinant?
    What is the MW of the protein standard?

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    Answer

    Thank you for contacting us.

    The kit standard is purified from human plasma with >95% purity by SDS-PAGE. The MW is 190,000 Da (2 chains).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
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