Human Complement C3a des Arg ELISA Kit (ab133037)
Key features and details
- Sensitivity: 0.12 ng/ml
- Range: 0.313 ng/ml - 20 ng/ml
- Sample type: Plasma
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Human Complement C3a des Arg ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Low conc. 16 1.86ng/ml 8.7% Medium conc. 16 3.4ng/ml 9.8% High conc. 16 12.3ng/ml 11.1% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Low 0.753ng/ml 5.7% Medium 1.529ng/ml 16.9% High 3.491ng/ml 28.6% -
サンプルの種類
Plasma -
アッセイタイプ
Competitive -
検出感度
0.12 ng/ml -
検出範囲
0.313 ng/ml - 20 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Plasma 94.8 % - % -
全工程の試験時間
3h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Complement C3a des Arg Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is for the accurate quantitation of Human Complement C3a des Arg in plasma.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Complement C3a des Arg antigen and a polyclonal rabbit antibody specific to Complement C3a des Arg. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Complement C3a des Arg captured in the plate.
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特記事項
The Human C3a des Arg molecule is one of three activation fragments formed from the activation of the complement cascade. C3a des Arg is formed from C3a via carboxypeptidase cleavage of the C-terminal arginine group. The structure of Human C3a des Arg was first reported in 1975 by Hugli. Human C3a des Arg contains 77 amino acids with 6 cysteines involved in disulfide bridges between residues 22-49, 23-56 and 36-57. The C terminal end of C3a des Arg in Human, porcine, rat, mouse and guinea pig is identical. C3a des Arg is a highly cationic molecule containing no carbohydrate. X-ray crystal data shows the N and C terminal residues to have highly flexible helical structures. C3a is one of the most potent constrictors of smooth muscle cells, and guinea pig airways are hyperresponsive to C3a when pretreated with histamine. The long term study of liver and other transplant recipients for both C3a des Arg and C4a des Arg may be useful in assessing a number of pathological conditions. The use of potent protease inhibitors, such as Futhan, in conjunction with EDTA, may allow complement activation factors to be quantitated specifically via inhibition of non-specific protease formation of C3a des Arg.
Cross Reactivity
Compound Cross Reactivity Human Complement C3a des Arg
100% Human Complement C3
1.28% Human Complement C4a des Arg
0.40% Human Complement C4 0.31% Human Complement C5 des Arg
0.10% Human Complement C5 0.05% Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer Concentrate 1 x 30ml Assay Buffer 10 Concentrate 1 x 15ml Complement Reagent A 1 x 15ml Complement Reagent B 1 x 30ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Human Complement C3a des Arg EIA Antibody 1 x 6ml Human Complement C3a des Arg EIA Conjugate 1 x 6ml Human Complement C3a des Arg Standard 2 x 500ng Plate Sealer 2 units pNpp Substrate 1 x 23ml Stop Solution 1 x 5ml -
研究分野
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関連性
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
細胞内局在
Secreted -
別名
- C3
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
- Complement C3
- CPAMD1
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参照データベース
- Entrez Gene: 718 Human
- Omim: 120700 Human
- SwissProt: P01024 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab133037 は 5 報の論文で使用されています。
- Kefalogianni R et al. Complement activation during cardiopulmonary bypass and association with clinical outcomes. EJHaem 3:86-96 (2022). PubMed: 35846208
- Zhu X et al. All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-IL-1ß loop. Haematologica N/A:N/A (2019). PubMed: 30679324
- Onwukwe C et al. Engineering Intravenously Administered Nanoparticles to Reduce Infusion Reaction and Stop Bleeding in a Large Animal Model of Trauma. Bioconjug Chem 29:2436-2447 (2018). PubMed: 29965731
- Hickman DA et al. Intravenous synthetic platelet (SynthoPlate) nanoconstructs reduce bleeding and improve 'golden hour' survival in a porcine model of traumatic arterial hemorrhage. Sci Rep 8:3118 (2018). PubMed: 29449604
- Zhang X & Sun L Anaphylatoxin C3a: A potential biomarker for esophageal cancer diagnosis. Mol Clin Oncol 8:315-319 (2018). PubMed: 29435296