Human Complement C1q ELISA Kit (ab170246)
Key features and details
- Sensitivity: 64 pg/ml
- Range: 0.078 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Complement C1q ELISA Kit
C1q キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Plasma 5.1% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Plasma 9.9% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
> 64 pg/ml -
検出範囲
0.078 ng/ml - 5 ng/ml -
添加回収試験
= 96 %
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全工程の試験時間
4h 00m -
種交差性
交差種: Human
非交差種: Mouse, Rat, Rabbit, Cow, Pig -
製品の概要
Complement C1q Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab170246) is designed for the quantitative measurement of Complement C1q concentrations in plasma, serum, saliva, urine, milk, cerebrospinal fluid and cell culture supernatants.
A Complement C1q specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C1q specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C1q captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Complement C1q Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Complement C1q Microplate (12 x 8 well strips) 1 unit Complement C1q Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
関連疾患
Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
配列類似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
翻訳後修飾
O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C1qa
- C1QA_HUMAN
- C1QB
see all -
参照データベース
- Entrez Gene: 713 Human
- Entrez Gene: 714 Human
- Entrez Gene: 712 Human
- Omim: 120550 Human
- Omim: 120570 Human
- Omim: 120575 Human
- SwissProt: P02746 Human
- SwissProt: P02747 Human
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab170246 は 15 報の論文で使用されています。
- van der Ende EL et al. A data-driven disease progression model of fluid biomarkers in genetic frontotemporal dementia. Brain 145:1805-1817 (2022). PubMed: 34633446
- Choi YH et al. Natural antibody IgG levels are associated with HBeAg-positivity and seroconversion in chronic hepatitis B patients treated with entecavir. Sci Rep 12:4382 (2022). PubMed: 35288624
- Kim D et al. Old plasma dilution reduces human biological age: a clinical study. Geroscience 44:2701-2720 (2022). PubMed: 35999337
- van der Ende EL et al. Elevated CSF and plasma complement proteins in genetic frontotemporal dementia: results from the GENFI study. J Neuroinflammation 19:217 (2022). PubMed: 36064709
- Luo X et al. Plasma complement C3 and C3a are increased in major depressive disorder independent of childhood trauma. BMC Psychiatry 22:741 (2022). PubMed: 36447174