Human CNTF ELISA Kit (ab99994)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.823 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human CNTF ELISA Kit
CNTF キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.2 ng/ml -
検出範囲
0.823 ng/ml - 200 ng/ml -
添加回収試験
> 92 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 95.45 84% - 104% Plasma 93.28 83% - 104% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human
非交差種: Rat -
製品の概要
Abcam’s CNTF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CNTF in plasma and cell culture supernatants. (Human CNTF concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human CNTF in normal serum with ab99994, only in serum spiked with Human CNTF.
-
特記事項
Optimization may be required with urine samples.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CNTF 2 vials CNTF Microplate (12 strips x 8 wells) 1 unit Recombinant Human CNTF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
関連性
Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells. -
細胞内局在
Cytoplasmic -
別名
- AI429687
- Ciliary Neurotrophic Factor
- HCNTF
see all -
参照データベース
- Entrez Gene: 1270 Human
- Omim: 118945 Human
- SwissProt: P26441 Human
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab99994 は 1 報の論文で使用されています。
- Cen LP et al. Human Periodontal Ligament-Derived Stem Cells Promote Retinal Ganglion Cell Survival and Axon Regeneration After Optic Nerve Injury. Stem Cells 36:844-855 (2018). PubMed: 29476565