Human Chemerin ELISA Kit (ab155430)
Key features and details
- Sensitivity: 0.5 ng/ml
- Range: 0.51 ng/ml - 50 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Chemerin ELISA Kit
Chemerin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% overall < 10% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% overall < 12% -
サンプルの種類
Serum, Plasma, Cell culture media -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 0.5 ng/ml -
検出範囲
0.51 ng/ml - 50 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum = 126.3 107% - 145% Plasma = 134.6 105% - 144% Cell culture media = 136.9 120% - 146% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Chemerin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Chemerin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Chemerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Chemerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Chemerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Chemerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimisation may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human Chemerin 2 vials Chemerin Microplate (12 x 8 wells) 1 unit Recombinant Human Chemerin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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関連性
This gene encodes a secreted chemotactic protein that initiates chemotaxis via the ChemR23 G protein-coupled seven-transmembrane domain ligand. Expression of this gene is upregulated by the synthetic retinoid tazarotene and occurs in a wide variety of tissues. The active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein. -
細胞内局在
Secreted -
別名
- chemerin
- HP10433
- RAR-responsive protein TIG2
see all -
参照データベース
- Entrez Gene: 5919 Human
- Omim: 601973 Human
- SwissProt: Q99969 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab155430 は 6 報の論文で使用されています。
- Oldfield L et al. Blood levels of adiponectin and IL-1Ra distinguish type 3c from type 2 diabetes: Implications for earlier pancreatic cancer detection in new-onset diabetes. EBioMedicine 75:103802 (2022). PubMed: 34990893
- Mir MM et al. Differential Association of Selected Adipocytokines, Adiponectin, Leptin, Resistin, Visfatin and Chemerin, with the Pathogenesis and Progression of Type 2 Diabetes Mellitus (T2DM) in the Asir Region of Saudi Arabia: A Case Control Study. J Pers Med 12:N/A (2022). PubMed: 35629157
- Tretjakovs P et al. Thioredoxin-1 and Correlations of the Plasma Cytokines Regarding Aortic Valve Stenosis Severity. Biomedicines 9:N/A (2021). PubMed: 34440245
- Song F et al. Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients. Front Endocrinol (Lausanne) 11:177 (2020). PubMed: 32300333
- Léniz A et al. Chemerin concentrations in infants born small for gestational age: correlations with triglycerides and parameters related to glucose homeostasis. J Physiol Biochem N/A:N/A (2020). PubMed: 32557228
- Fontes VS et al. Analysis of the chemerin and resistin adipokines in children and adolescents. Rev Assoc Med Bras (1992) 66:300-306 (2020). PubMed: 32520149