Human CEA ELISA Kit (ab264604)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24.68 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture extracts, Cell culture media, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CEA ELISA Kit
CEACAM5 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% extract 8 5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% extract 3 5.4% -
サンプルの種類
Serum, Cell culture extracts, Tissue Extracts, Cell Lysate, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
24.68 pg/ml -
検出範囲
78.13 pg/ml - 5000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 100 92% - 116% Cell culture extracts 108 95% - 122% Tissue Extracts 109 104% - 117% Cell culture media 93 84% - 99% Hep Plasma 101 90% - 110% EDTA Plasma 98 91% - 110% Cit plasma 105 91% - 111% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human CEA ELISA Kit (ab264604) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CEA protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, cell culture extracts, cell lysate, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CEA with 24.68 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
CEA is encoded by the CECAM5 gene which produces a cell adhesion molecule consisting of an Ig variable region-like amino-terminal domain followed by up to six Ig constant region-like domains anchored to the cell membrane by either a glycosylphosphatidylinositol (GPI) moiety or a proteinaceous transmembrane and cytoplasmic domain. CEA is normally expressed on epithelial cells and is found abundantly on the apical surface of the gastrointestinal epithelium, as well as the nasopharynx, lung, urogenital tract, and sweat glands. Under physiologic conditions, only low amounts of soluble CEA can be detected in serum. However, in many different cancers, CEA is highly up regulated.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human CEA Capture Antibody 1 x 600µl 10X Human CEA Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CEA Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. -
組織特異性
Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. -
配列類似性
Belongs to the immunoglobulin superfamily. CEA family.
Contains 7 Ig-like (immunoglobulin-like) domains. -
翻訳後修飾
Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. -
細胞内局在
Cell membrane. - Information by UniProt
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別名
- Carcinoembryonic antigen
- Carcinoembryonic antigen-related cell adhesion molecule 5
- CD66e
see all -
参照データベース
- Entrez Gene: 1048 Human
- Omim: 114890 Human
- SwissProt: P06731 Human
- Unigene: 709196 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Standard Curve comparison between human CEA SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Standard Curve comparison between ab264604 and ab183365 SimpleStep ELISA kits. ab264604 SimpleStep ELISA kit shows increased sensitivity.
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The CEA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of CEA were measured in duplicate and interpolated from the CEA standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEA concentration was determined to be 3,584.91 pg/ml in MCF7 and 3,812.56 pg/mL in colon homogenate extract.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEA concentration was determined to be 1,027.57 pg/mL with a range of ND – 4,257.59 pg/mL.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab264604 は 2 報の論文で使用されています。
- Chen Y et al. Boronate ester bond-based potentiometric aptasensor for screening carcinoembryonic antigen-glycoprotein using nanometer-sized CaCO3 with ion-selective electrode. Anal Bioanal Chem 413:1073-1080 (2021). PubMed: 33230701
- Chi L et al. Thionine-doped nanometer-sized silica conjugated with phenylboronic acid: An innovative recognition/signal element for voltammetric aptasensing of colorectal cancer-related carcinoembryonic antigen. Anal Chim Acta 1136:91-98 (2020). PubMed: 33081954