Human CD85d ELISA Kit (LILRB2) (ab269551)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15.9 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CD85d ELISA Kit (LILRB2)
ILT-4 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 2.1% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 5.6% -
サンプルの種類
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
15.9 pg/ml -
検出範囲
62.5 pg/ml - 4000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 91 100% - 95% Hep Plasma 84 93% - 89% EDTA Plasma 102 94% - 107% Cit plasma 95 100% - 94% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human CD85d ELISA Kit (LILRB2) (ab269551) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD85d (LILRB2) protein in edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD85d (LILRB2) with 15.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
CD85d (LILRB2), Leukocyte immunoglobulin-like receptor subfamily B member 2, is a key protein in the regulation of the immune response where it plays a pivotal as a negative regulator. It is primarily found on immune cells where it interacts with MHC domains on antigen presenting cells. Given its important role in immunosuppression, it is currently under investigation as a drug-targetable protein for a host of disease states. The antibodies for this kit were raised against a recombinant protein fragment targeting the extracellular domain of this receptor. There is little homology between mouse and rat for this fragment, so reactivity is not likely.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human CD85d (LILRB2) Capture Antibody 1 x 600µl 10X Human CD85d (LILRB2) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CD85d (LILRB2) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance. Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. -
組織特異性
Expressed on monocytes and B-cells, and at lower levels on dendritic cells. Detected at low levels in natural killer (NK) cells. -
配列類似性
Contains 4 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
Contains 3 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻訳後修飾
Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6. -
細胞内局在
Membrane. - Information by UniProt
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別名
- CD85 antigen-like family member D
- CD85d
- CD85d antigen
see all -
参照データベース
- Entrez Gene: 10288 Human
- Omim: 604815 Human
- SwissProt: Q8N423 Human
- Unigene: 655652 Human
関連製品
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Alternative Versions
画像
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The CD85d standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of CD85d were measured in duplicates, interpolated from the CD85d standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 30%, plasma (EDTA) 30%, plasma (citrate) 30%, and plasma (heparin) 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD85d concentration was determined to be 8.85 ng/mL in serum, 6.75 ng/mL in plasma (EDTA), 8.82 ng/mL in plasma (citrate), and 7.72 ng/mL in plasma (Heparin).
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab269551 は論文での使用が確認できていません。