Human CD31 ELISA Kit (PECAM) (ab190814)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 44 pg/ml
- Range: 226.6 pg/ml - 14500 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Rhesus monkey
製品の概要
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製品名
Human CD31 ELISA Kit (PECAM)
CD31 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% cell extract 8 3.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% cell extract 3 3.3% -
サンプルの種類
Cell culture supernatant, Saliva, Urine, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
44 pg/ml -
検出範囲
226.6 pg/ml - 14500 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Urine 80 76% - 84% Serum 113 109% - 119% Hep Plasma 74 71% - 80% EDTA Plasma 103 102% - 106% Cit plasma 91 88% - 95% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human, Rhesus monkey
非交差種: Cow -
製品の概要
Human CD31 ELISA Kit (PECAM) (ab190814) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD31 (PECAM) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD31 (PECAM) with 44 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
CD31, also known as PECAM-1, is a cell adhesion molecule which is required for leukocyte trans-endothelial migration (TEM) under most inflammatory conditions. CD31 is a 130-kDa glycoprotein constitutively expressed on endothelial cells, platelets, neutrophils, monocytes, lymphocytes and basophils. CD31 molecules is expressed in large amounts in the intracellular junctions of endothelial cells. Experiments using antibody results in blocking CD31 homophilic interaction and inhibition of leukocytes transmigration. Expression of CD31 on the cell surface is regulated by receptor shedding resulting in generation of a soluble 100-kDa extracellular domain form and a truncated 28-kDa fragment containing the transmembrane and cytoplasmic domain of CD31.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human CD31 Capture Antibody 1 x 600µl 10X Human CD31 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 2 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Denaturant 1 x 500µl Human CD31 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
組織特異性
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
配列類似性
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
翻訳後修飾
Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
細胞内局在
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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別名
- Adhesion molecule
- CD31
- CD31 antigen
see all -
参照データベース
- Entrez Gene: 5175 Human
- Omim: 173445 Human
- SwissProt: P16284 Human
- Unigene: 376675 Human
- Unigene: 514412 Human
関連製品
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Alternative Versions
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ELISA kits
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Standard Curve comparison between human CD31 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Standard Curve comparison between human CD31 new SimpleStep ELISA kit and previous ELISA kit. The new SimpleStep ELISA kit shows increased sensitivity.
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The CD31 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The CD31 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 36%, plasma (citrate) 9%, plasma (heparin) 9% and plasma (EDTA) 9%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 6.60 ng/mL in neat serum, 5.04 ng/mL in neat plasma (citrate), 5.67 ng/mL in neat plasma (heparin), and 6.44 ng/mL in neat plasma (EDTA).
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THP-1 cells were cultured in the presence (stimulated) or absence (mock) of 5 µg/mL LPS and 50nM PMA for 24 hours. The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 36%, urine 36%, THP-1 stimulated supernatant 18%, and THP-1 mock supernatant 18%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 6.85 ng/mL in neat saliva, 0.81 ng/mL in neat urine, 2.27 ng/mL in neat stimulated THP-1 supernatant, and 0.64 ng/mL in neat mock THP-1 supernatant.
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THP-1 cells were cultured in the presence (stimulated) or absence (unstimulated) of 5 µg/mL LPS and 50nM PMA for 24 hours. The concentrations of CD31 were measured in duplicate and interpolated from the CD31 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 16.0 ng/mL in stimulated THP-1 cell extract and 12.5 ng/mL in unstimulated THP-1 cell extract.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 6.34 ng/mL with a range of 4.39 – 11.1 ng/mL
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab190814 は 2 報の論文で使用されています。
- Li W et al. Intermittent hypoxia-induced downregulation of microRNA-320b promotes lung cancer tumorigenesis by increasing CDT1 via USP37. Mol Ther Nucleic Acids 24:528-541 (2021). PubMed: 33898105
- Meng D et al. Effects of VEGFR1+ hematopoietic progenitor cells on pre-metastatic niche formation and in vivo metastasis of breast cancer cells. J Cancer Res Clin Oncol 145:411-427 (2019). PubMed: 30483898