Human CD200R ELISA Kit (CD200R1) (ab272196)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.89 pg/ml
- Range: 46.88 pg/ml - 3000 pg/ml
- Sample type: Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CD200R ELISA Kit (CD200R1)
CD200R キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 7.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 7.7% -
サンプルの種類
Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
11.89 pg/ml -
検出範囲
46.88 pg/ml - 3000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 115 112% - 120% Cell Lysate 99 90% - 116% Hep Plasma 91 81% - 99% EDTA Plasma 93 81% - 110% Cit plasma 92 83% - 109% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human CD200R ELISA kit (ab272196) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD200R protein in serum, plasma and cell and tissue culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD200R with 11.89 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
CD200R (CD200R1), is a transmembrane glycoprotein member of the immunoglobin superfamily. CD200R acts as an inhibitory receptor for the CD200/OX-2 cell surface glycoprotein. CD200R is mainly expressed in myeloid cells and CD4+ T-cells and known to interact with CD200 in variety of cells including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells. Activation of CD200R regulates the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase. Recent studies suggest that CD200R may prove useful in monitoring changes in bone metabolism. Mouse, rat and cow share 57%, 57% and 60% sequence homology with human CD200R.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human CD200R (CD200R1) Capture Antibody 1 x 600µl 10X Human CD200R (CD200R1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human CD200R (CD200R1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. -
組織特異性
Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells. -
配列類似性
Belongs to the CD200R family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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別名
- CD 200R
- CD200 cell surface glycoprotein receptor
- CD200 receptor 1
see all -
参照データベース
- Entrez Gene: 131450 Human
- Omim: 607546 Human
- SwissProt: Q8TD46 Human
- Unigene: 309158 Human
関連製品
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Alternative Versions
画像
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The CD200R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of CD200R were measured in duplicates, interpolated from the CD200R standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD200R concentration was determined to be 2,595 pg/mL in serum, 2,460 pg/mL in plasma (citrate), 2,904 pg/mL in plasma (EDTA), and 3,137 pg/mL in plasma (heparin).
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The concentrations of CD200R were measured in duplicate and interpolated from the CD200R standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD200R concentration was determined to be 817.1 pg/mL in HEL cell extract sample.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272196 は論文での使用が確認できていません。