Human CCL16 ELISA Kit (ab243673)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 2.34 pg/ml - 150 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CCL16 ELISA Kit
LEC キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% sample 8 1.9% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% sample 3 4.7% -
サンプルの種類
Cell culture supernatant, Serum, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1 pg/ml -
検出範囲
2.34 pg/ml - 150 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 110 103% - 121% Serum 110 81% - 128% Cit plasma 97 80% - 117% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human
非交差種: Cow -
製品の概要
Human CCL16 ELISA Kit (ab243673) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL16 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL16 with 1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human CCL16 Capture Antibody 1 x 600µl 10X Human CCL16 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human CCL16 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES. -
組織特異性
Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma-activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C-C motif chemokine 16
- CCL16
- CCL16_HUMAN
see all -
参照データベース
- Entrez Gene: 6360 Human
- Omim: 601394 Human
- SwissProt: O15467 Human
- Unigene: 10458 Human
関連製品
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Alternative Versions
画像
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The CCL16 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of CCL16 were measured in duplicates, interpolated from the CCL16 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1.5%, and HepG2 supernatant 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL16 concentration was determined to be 15284.9 pg/mL in serum, 12472.9 pg/mL in plasma (citrate) and 599.3 pg/mL in HepG2 supernatant.
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To learn more about the advantages of recombinant antibodies see here.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab243673 は論文での使用が確認できていません。