Human CCL14 ELISA Kit (HCC-1) (ab272201)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.4 pg/ml
- Range: 5.5 pg/ml - 350 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human CCL14 ELISA Kit (HCC-1)
CCL14 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 3.6% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 4 5.1% -
サンプルの種類
Saliva, Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
4.4 pg/ml -
検出範囲
5.5 pg/ml - 350 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Saliva 88 83% - 94% Urine 101 99% - 102% Serum 111 108% - 118% Cell culture media 98 95% - 100% Hep Plasma 102 101% - 104% EDTA Plasma 112 109% - 117% Cit plasma 112 108% - 119% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human CCL14 ELISA kit (ab272201) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL14 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL14 with 4.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
C-C motif chemokine 14 (CCL14), also known as HCC-1 and NCC-2, is a cytokine encoded by the gene CCL14. Mature secreted CCL14 is a 74- amino acid containing active glycoprotein and is constitutively expressed by multiple tissues and has been found at high concentrations in plasma.
CCl14 enhances the proliferation of CD34 myeloid progenitor cells and has weak activities on human monocytes through receptors that also recognize MIP-1 alpha. CCL14 induces intracellular Ca2+ changes and enzyme release but does not induce chemotaxis in monocytes.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human CCL14 (HCC-1) Capture Antibody 1 x 600µl 10X Human CCL14 (HCC-1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human CCL14 (HCC-1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. -
組織特異性
Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood.
HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CC-1
- CC-3
- CCL14
see all -
参照データベース
- Entrez Gene: 6358 Human
- Omim: 601392 Human
- SwissProt: Q16627 Human
- Unigene: 272493 Human
- Unigene: 740501 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CCL14 standard curve in Sample Diluent NS.The CCL14 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CCL14 in human serum, plasma (EDTA), plasma (citrate), plasma (heparin).The concentrations of CCL14 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (EDTA) 1:200, plasma (citrate) 1:200, plasma (heparin) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 59.0 ng/mL in serum, 43.1 ng/mL in plasma (EDTA), 48.8 ng/mL in and plasma (citrate), and 98.9 ng/mL in plasma (heparin).
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Interpolated concentrations of native CCL14 in human saliva and urine.The concentrations of CCL14 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum, plasma (EDTA), plasma – (citrate), plasma – (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 96.7 pg/mL in saliva and 87.9 pg/ml in urine.
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Serum from eight individual healthy CCL14 donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL14 concentration was determined to be 33.3 ng/mL with a range of 20.5 – 56.5 ng/mL.
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Sandwich ELISA - Human CCL14 ELISA Kit (HCC-1) (ab272201)To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272201 は論文での使用が確認できていません。