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Human Cathepsin B ELISA Kit (ab272205)

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SimpleStep ELISA Protocol Diagram
  • Human Cathespin B competitor curve comparison
  • Example of human Cathepsin B standard curve in Sample Diluent NS.
  • Interpolated concentrations of native Cathepsin B in human serum and plasma samples.
  • Interpolated concentrations of native Cathepsin B in treated human PBMC cell culture supernatant and male saliva samples.
  • Serum from ten individual healthy human male donors was measured in duplicate.
  • Sandwich ELISA - Human Cathepsin B ELISA Kit (ab272205)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 23.44 pg/ml
  • Range: 109.38 pg/ml - 7000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human Cathepsin B ELISA Kit
    Cathepsin B キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Serum 8 4.8%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Serum 3 6%

  • サンプルの種類

    Cell culture supernatant, Saliva, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    23.44 pg/ml

  • 検出範囲

    109.38 pg/ml - 7000 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 100 87% - 106%
    Saliva 108 95% - 108%
    Urine 91 84% - 100%
    Serum 98 95% - 99%
    Hep Plasma 103 92% - 113%
    EDTA Plasma 114 110% - 122%
    Cit plasma 93 88% - 101%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human Cathepsin B ELISA kit (ab272205) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin B protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cathepsin B with 41.8 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less


            -High sensitivity, specificity and reproducibility from superior antibodies


            -Fully validated in biological samples


            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    Cathepsin B is a part of a family of cysteine lysosomal proteases. Cathepsin B is synthesized as a preproenzyme in the endoplasmic reticulum and activated after transportation to the Golgi apparatus. Cathepsin B is overexpressed in invasive and metastatic cancers and is such a potential biomarker for a variety of cancers. Double knockout of cathepsin B and cathepsin L in mice is lethal due to their role in the central nervous system.

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

画像

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Human Cathespin B competitor curve comparison
    Human Cathespin B competitor curve comparison

    Standard Curve comparison between Human Cathepsin B SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Example of human Cathepsin B standard curve in Sample Diluent NS.
    Example of human Cathepsin B standard curve in Sample Diluent NS.

    The Cathepsin B standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Cathepsin B in human serum and plasma samples.
    Interpolated concentrations of native Cathepsin B in human serum and plasma samples.

    The concentrations of Cathepsin B were measured in duplicates, interpolated from the Cathepsin B standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 8%, plasma (citrate) 10%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 54.38 ng/mL in serum, 56.17 ng/mL in plasma (citrate), 55.98 ng/ml in plasma (heparin), 55.79 ng/ml in plasma (EDTA).

  • Interpolated concentrations of native Cathepsin B in treated human PBMC cell culture supernatant and male saliva samples.
    Interpolated concentrations of native Cathepsin B in treated human PBMC cell culture supernatant and male saliva samples.

    PBMC supernatant samples were treated with 1.5% PHA-M for 46 hours. The concentrations of Cathepsin B were measured in duplicates, interpolated from the Cathepsin B standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 4% and saliva 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 103.07 ng/mL in treated PBMC supernatant and 7.00 ng/mL in male saliva.

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 98.94 ng/mL with a range of 54.68 – 219.68 ng/mL.

  • Sandwich ELISA - Human Cathepsin B ELISA Kit (ab272205)
    Sandwich ELISA - Human Cathepsin B ELISA Kit (ab272205)
    To learn more about the advantages of recombinant antibodies see here.

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