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Human Anti-Candida albicans IgA ELISA Kit (ab108714)

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Serologic ELISA assay principle

    Key features and details

    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Indirect
    • Reacts with: Human

    製品の概要

    • 製品名

      Human Anti-Candida albicans IgA ELISA Kit

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Pos.Serum 10 6.6%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Pos.Serum 33 9%

    • サンプルの種類

      Serum, Hep Plasma, Cit plasma

    • アッセイタイプ

      Indirect

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s anti-Candida albicans IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Candida albicans in Human serum and plasma.


      A 96-well plate has been precoated with Candida albicans antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Candida albicans-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Candida albicans IgA sample captured in plate.

    • 試験プラットフォーム

      Microplate

    製品の特性

    画像

    • Serologic ELISA assay principle
      Serologic ELISA assay principle
      Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.

    参考文献 (1)

    ab108714 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108714 は 1 報の論文で使用されています。

    • Wu G  et al. AGO1 enhances the proliferation and invasion of cholangiocarcinoma via the EMT-associated TGF-ß signaling pathway. Am J Transl Res 12:2890-2902 (2020). PubMed: 32655817

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