Human Anti-Borrelia burgdorferi IgM ELISA Kit (ab108711)
Key features and details
- Sample type: Cerebral Spinal Fluid, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
製品の概要
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製品名
Human Anti-Borrelia burgdorferi IgM ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Pos. Serum 8 8.6% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Pos. Serum 14 4.1% -
サンプルの種類
Serum, Hep Plasma, Cit plasma, Cerebral Spinal Fluid -
アッセイタイプ
Indirect -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s anti-Borrelia burgdorferi IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF (cerebrospinal fluid).
A 96-well plate has been precoated with recombinant Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgM sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Borrelia burgdorferi (IgM) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Borrelia burgdorferi anti-IgM HRP Conjugate Red, Black cap 1 x 20ml Borrelia burgdorferi IgM Cut-off Control Yellow, Green cap 1 x 3ml Borrelia burgdorferi IgM Negative Control Yellow, Blue cap 1 x 2ml Borrelia burgdorferi IgM Positive Control Yellow, Red cap 1 x 2ml Cover foil 1 unit IgM Sample Diluent Green, White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究分野
画像
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108711 は 1 報の論文で使用されています。
- Elhelw RA et al. Lyme borreliosis: A neglected zoonosis in Egypt. Acta Trop 140:188-92 (2014). PubMed: 25239124