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Human Beta Nerve Growth Factor ELISA Kit (NGFB) (ab99986)

Reviews (6)Q&A (13)References (10)
Typical Standard Curve

    Key features and details

    • Sensitivity: 14 pg/ml
    • Range: 6.86 pg/ml - 5000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    製品の概要

    • 製品名

      Human Beta Nerve Growth Factor ELISA Kit (NGFB)
      Beta nerve growth factor キット 製品一覧

    • 検出方法

      Colorimetric

    • サンプルの種類

      Cell culture supernatant, Serum, Plasma

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      < 14 pg/ml

    • 検出範囲

      6.86 pg/ml - 5000 pg/ml

    • 添加回収試験

      > 100 %

      特定サンプルでの回収試験
      サンプルの種類 平均 % 測定範囲
      Cell culture supernatant 106.7 90% - 122%
      Serum 113.1 98% - 116%
      Plasma 102.8 85% - 117%

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s Beta Nerve Growth Factor (NGFB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Beta Nerve Growth Factor in serum, plasma, and cell culture supernatants.


      This assay employs an antibody specific for Human Beta Nerve Growth Factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Beta Nerve Growth Factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Beta Nerve Growth Factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Beta Nerve Growth Factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


      Get results in 90 minutes with Human beta Nerve Growth Factor ELISA Kit (ab193760) from our SimpleStep ELISA® range.

    • 特記事項

      NGFB concentration is very low in normal human serum samples and may be undetectable even with undiluted samples. Optimization may be required with urine samples.

    • 試験プラットフォーム

      Microplate

    製品の特性

    • 保存方法

      Store at -20°C. Please refer to protocols.
    • 内容 1 x 96 tests
      20X Wash Buffer 1 x 25ml
      5X Assay Diluent 1 x 15ml
      800X HRP-Streptavidin Concentrate 1 x 200µl
      Beta Nerve Growth Factor Microplate (12 x 8 wells) 1 unit
      Beta Nerve Growth Factor Standard Human (recombinant) (lyophilized) 2 vials
      Biotinylated anti-Human Beta Nerve Growth Factor 2 vials
      Stop Solution 1 x 8ml
      TMB One-Step Substrate Reagent 1 x 12ml

    • 研究分野

    • 関連性

      Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.

    • 細胞内局在

      Secreted.

    • 別名

      • Beta nerve growth factor
      • Beta NGF
      • HSAN5
      • MGC161426
      • MGC161428
      • Nerve growth factor (beta polypeptide)
      • Nerve growth factor beta polypeptide
      • Nerve growth factor beta subunit
      • NGFB
      • NID67
      see all

    • 参照データベース

      関連製品

      画像

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab99986.

      参考文献 (10)

      ab99986 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab99986 は 10 報の論文で使用されています。

      • Kumar A  et al. Localization and hypersecretion of nerve growth factor in breast phyllodes tumors: Evidence from a preliminary study. Cancer Rep (Hoboken) 4:e1300 (2021). PubMed: 33026185
      • Bioque M  et al. Acute effects of a session of electroconvulsive therapy on brain-derived neurotrophic factor plasma levels. Rev Psiquiatr Salud Ment N/A:N/A (2020). PubMed: 32674992
      • Cen LP  et al. Human Periodontal Ligament-Derived Stem Cells Promote Retinal Ganglion Cell Survival and Axon Regeneration After Optic Nerve Injury. Stem Cells 36:844-855 (2018). PubMed: 29476565
      • Shi W  et al. TrkA is a binding partner of NPM-ALK that promotes the survival of ALK+ T-cell lymphoma. Mol Oncol 11:1189-1207 (2017). PubMed: 28557340
      • Saeednia S  et al. Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation. Reprod Biol Endocrinol 14:29 (2016). PubMed: 27233989
      • Gilbert HT  et al. Acidic pH promotes intervertebral disc degeneration: Acid-sensing ion channel -3 as a potential therapeutic target. Sci Rep 6:37360 (2016). ELISA ; Human . PubMed: 27853274
      • Fontanilla CV  et al. Adipose-derived Stem Cell Conditioned Media Extends Survival time of a mouse model of Amyotrophic Lateral Sclerosis. Sci Rep 5:16953 (2015). ELISA . PubMed: 26586020
      • Shimko MJ  et al. Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiol Rep 2:N/A (2014). PubMed: 25347857
      • Li YJ  et al. Serum nerve grow factor and brain-derived neurotrophic factor profiles in Sjögren's syndrome concomitant with interstitial lung disease. Clin Rheumatol 33:1161-4 (2014). PubMed: 24691584
      • de Almeida JF  et al. Stem cells of the apical papilla regulate trigeminal neurite outgrowth and targeting through a BDNF-dependent mechanism. Tissue Eng Part A 20:3089-100 (2014). PubMed: 24837134

      レビューと Q&A

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      1-10 of 19 Abreviews or Q&A

      Trial with equine serum, plasma and synovial fluid

       Inconclusive Excellent 5/5 (Ease of Use)
      Abreviews
      abreview image
      The kit performance for equine samples was tested. Specifically, dilution series from undiluted samples down to 1:128 dilution were run in duplicate. Two plasma samples and two serum samples from each of two individuals and one synovial sample were run. The kit was easy to use and the instructions clear. Unfortunately the equine NGF was not detected by the kit hence this kit can not be recommended for analysis of equine samples. Obtained OD values in all sample wells were below the level of detection.
      The standard dilutions gave a linear curve down to the final dilution. After contacting the support, we were recommended to remove the final standard dilution from the curve, as this is sometimes below the level of quantification in this kit.
      We have had excellent and professional support from the technical staff throughout the process, from before ordering to the end of the data interpretation.
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jul 08 2020

      Abreview for Beta NGF Human ELISA Kit

       Average Good 4/5 (Ease of Use)
      Abreviews
      The kit is easy to use,
      We got a good standard curve and R2 value, however its not sensitive to the serum samples.
      Some study reported that NGF level in ng/ml, but we can't detected through this kit. Why?
      Could you give me the solution. Thanks!
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jan 24 2018

      good

       Good Good 4/5 (Ease of Use)
      Abreviews
      Sample:Human Serum
      We got good results: Good standard curve and R2 value, enough signal.
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Sep 14 2017

      Validation of human B-NGF ELISA for sera of dromedary camesl

       Excellent Good 4/5 (Ease of Use)
      Abreviews
      Sera of dromedary camels have been validated for the determination of b-NGF using Abcam human b-NGF ELISA kit (ab99986).
      Serum from female dromedaries with suspected high serum b-NGF concentration was pooled. Then this serum was subjected to serial dilution (1:2). The dilution was carried out either in Assay Diluent (1x) provided by the manufacturer, or in pool of camel serum with low (undetectable) serum b-NGF concentration. The dilution has been done the same way as the preparation of the standard described in the protocol of the kit. Then, the assay was performed and optical density values have been plotted against dilution.
      Date obtained are in the table below.
      Standard Concentration Dilution Standard High CS in AD High CS in low CS
      ST 1 0 8 0.08 0.07 0.07
      ST 2 20.58 7 0.14 0.17 0.14
      ST 3 61.7 6 0.25 0.31 0.26
      ST 4 185.2 5 0.54 0.65 0.55
      ST 5 555.6 4 1.25 1.35 1.22
      ST 6 1667 3 2.35 2.38 2.27
      ST 7 5000 2 3.32 3.2 3.15
      ST 8 10000 1 3.65 3.57 3.54
      In conclusion, b-NGF in camel sera after serial dilution showed an excellent parallelism compared to the standard (recombinant human b-NGF diluted in Assay Diluent). The kit is suitable for the determination of b-NGF in the serum of dromedary camels.
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Sep 13 2016

      The kit is not saliva sensitive

       Average Good 4/5 (Ease of Use)
      Abreviews
      abreview image
      The kit is easy to use,
      however its not sensitive to saliva samples
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Apr 08 2015

      Abreview for Beta NGF Human ELISA Kit

       Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      Sample: Pig Cell (bronchioaveolar lavage)
      Specification: bronchioaveolar lavage
      Amount of Sample: 500 µg
      Type of Kit Used: ELISA
      Positive control: porcine Hippocampus
      Did you use the Abcam Kit protocol?: Yes

      Additional data
      Additional Notes: It is really important to follow the recommended sample and standard volumes.
      Abcam response: We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received a free primary antibody of their choice for performing these tests. We wish to thank them for participating in our 100% Abreview testing discount program.
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Aug 07 2012

      Question

      our customer would like to know which is the expiry date of AB99986 (lot GR-56322).

      Read More
      Answer

      Thank you for contacting us.

      As stated on the datasheet, ab99986 may be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at –80ºC) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2 to 8°C.

      If the whole kit is stored at -20ºC, then it can be used within one year but avoid repeated freeze-thaw cycles.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Question

      i buy beta ngf human Elisa kit (ab99986) 9 month ago and kip that in -20 centigrade refrigerator.

      now we use kit but micro vial of hrp-streptavidin is empty.

      what do you think about this happening and do you have recommendation for me ?

      can i buy this item only and do test?

      i am looking for your answer.

      Read More
      Answer

      Thank you for alerting us to this issue.

      Do you have your original order number and lot number? Depending on which lot you received, the HRP-streptavidin may have contained 8 ul, so you should try spinning down the vial before opening and check to see if it is there.

      I may be able to send you an HRP-streptavidin, but could you please confirm the above information so I know which lot to send you?

      I look forward to your reply.

      Read More

      Question

      I just submitted my Abcam Review, and we ordered a new product today using the discount code.

      Read More
      Answer

      Thank you for letting me know and thank you for participating in the testing program.

      Please do not hesitate to let me know if there is anything I might be able to do for you in the future.

      Read More

      Question

      I just wanted to provide an update that we were able to detect a signal with our porcine samples (bronchioaveolar lavages). I assume it is NGF because we used porcine hippocampus, an area rich in NGF, as a positive control. Thank-you for your help and please let me know if I can provide any additional information.

      Read More
      Answer

      That is good news. Thank you for keeping me informed.

      Please do not hesitate to contact us if you need any advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      1-10 of 19 Abreviews or Q&A

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