Human BAFF ELISA Kit (TNFSF13B) (ab188391)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12.7 pg/ml
- Range: 0.08 ng/ml - 5 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human BAFF ELISA Kit (TNFSF13B)
BAFF キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 8 8.3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 3 8.8% -
サンプルの種類
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
12.7 pg/ml -
検出範囲
0.08 ng/ml - 5 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 111 105% - 115% Cell culture media 108 106% - 110% Hep Plasma 104 101% - 108% EDTA Plasma 105 98% - 115% Cit plasma 106 100% - 111% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human BAFF ELISA Kit (TNFSF13B) (ab188391) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BAFF (TNFSF13B) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BAFF (TNFSF13B) with 12.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
B-cell activating factor (BAFF) protein that is encoded by the TNFSF13B gene. BAFF protein is expressed in B-cell lineage cells which functions as a cytokine and is also a member of the tumor necrosis factor (TNF) family of ligands. The BAFF cytokine acts as a potent B-cell activator promoting the proliferation and differentiation of B-cells. This protein is expressed as a membrane-bound type II transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. The membrane bound form can be cleaved from the membrane, generating a soluble protein fragment. BAFF steady-state concentrations depend on B-cells and also on the expression of BAFF-binding receptors.
Inadequate levels of BAFF will fail to activate B-cells to produce enough immunoglobulin and will lead to immunodeficiency. Excessive level of BAFF causes abnormally high antibody production, results in systemic lupus erythmatosis, rheumatoid arthritis, and many other autoimmune diseases.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human BAFF Capture Antibody 1 x 600µl 10X Human BAFF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human BAFF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
組織特異性
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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別名
- ApoL related ligand TALL 1
- B cell Activating Factor
- B lymphocyte stimulator
see all -
参照データベース
- Entrez Gene: 10673 Human
- Omim: 603969 Human
- SwissProt: Q9Y275 Human
- Unigene: 525157 Human
- Unigene: 596110 Human
関連製品
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ELISA kits
画像
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Quantification of BAFF interpolated from standard curve and multiplied by dilution factor.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab188391 は 3 報の論文で使用されています。
- Hinterleitner M et al. Early Reconstitution of Antibody Secreting Cells after Allogeneic Stem Cell Transplantation. J Clin Med 11:N/A (2022). PubMed: 35012014
- Cao Y et al. BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF-?B signaling pathway in glomerular mesangial cells. Mol Med Rep 21:795-805 (2020). PubMed: 31974601
- Alkhairy OK et al. RAC2 loss-of-function mutation in 2 siblings with characteristics of common variable immunodeficiency. J Allergy Clin Immunol N/A:N/A (2014). ELISA ; Human . PubMed: 25512081