Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 43 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human AST ELISA Kit (Aspartate Aminotransferase)
Aspartate Aminotransferase キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 4.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 1.8% -
サンプルの種類
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
43 pg/ml -
検出範囲
312.5 pg/ml - 20000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 93 81% - 109% Serum 101 95% - 114% Cell culture extracts 92 88% - 94% Tissue Extracts 99 97% - 103% Hep Plasma 103 101% - 104% EDTA Plasma 93 91% - 96% Cit plasma 93 89% - 97% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human AST ELISA Kit (Aspartate Aminotransferase) (ab263881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AST (Aspartate Aminotransferase) protein in cell extracts, tissue extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human AST (Aspartate Aminotransferase) with 43 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Cytoplasmic aspartate aminotransaminase (AST), is a key enzyme in the regulation of glutamate levels. It catalyzes the reversible transfer of an a-amino group between aspartate and glutamate. Given its central role in amino acid metabolism, it plays a critical role in many biological functions. In a clinical context, AST levels in the serum are used as a marker for liver health. This kit targets the full-length protein.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human AST (Aspartate Aminotransferase) Capture Antibody 1 x 600µl 10X Human AST (Aspartate Aminotransferase) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human AST (Aspartate Aminotransferase) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Plays a key role in amino acid metabolism. -
配列類似性
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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別名
- AATC_HUMAN
- Aspartate aminotransferase
- Aspartate aminotransferase 1
see all -
参照データベース
- Entrez Gene: 2805 Human
- Omim: 138180 Human
- SwissProt: P17174 Human
- Unigene: 500756 Human
関連製品
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Alternative Versions
画像
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 58 ng/mL with a range of 27 – 136 ng/mL.
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The concentrations of AST were measured in duplicate and interpolated from the AST standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 14 ng/mL in human liver extract and 14 ng/mL in HepG2 cell extract.
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The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 13 ng/mL in HepG2 cell culture supernatant.
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The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 63 ng/mL in serum, 39 ng/mL in plasma (citrate), 52 ng/mL in plasma (EDTA). and 41 ng/mL in plasma (heparin).
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The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 62.7 ng/mL in serum, 38.7 ng/mL in plasma (citrate), 51.9 ng/mL in plasma (EDTA). and 41.0 pg/mL in plasma (heparin).
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The concentrations of AST were measured in duplicate and interpolated from the AST standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 13.5 ng/mL in human liver extract.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 58.1 ng/mL with a range of 26.6 – 135.6 ng/mL.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab263881 は 1 報の論文で使用されています。
- Shi Q et al. Co-Culture of Human Primary Hepatocytes and Nonparenchymal Liver Cells in the Emulate® Liver-Chip for the Study of Drug-Induced Liver Injury. Curr Protoc 2:e478 (2022). PubMed: 35790095