Human Arginase 1 ELISA Kit (ab230930)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17.77 pg/ml
- Range: 187.5 pg/ml - 12000 pg/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Arginase 1 ELISA Kit
Liver Arginase キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Tissue 8 2.2% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Tissue 3 4.4% -
サンプルの種類
Tissue Extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
17.77 pg/ml -
検出範囲
187.5 pg/ml - 12000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Tissue Extracts 103 99% - 107% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Arginase 1 ELISA Kit (ab230930) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Arginase 1 protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Arginase 1 with 17.77 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human Arginase 1 Capture Antibody 1 x 600µl 10X Human Arginase 1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Arginase 1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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パスウェイ
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
関連疾患
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
配列類似性
Belongs to the arginase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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別名
- A I
- Al
- ARG 1
see all -
参照データベース
- Entrez Gene: 383 Human
- Omim: 608313 Human
- SwissProt: P05089 Human
- Unigene: 440934 Human
関連製品
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Alternative Versions
画像
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Arginase 1 were measured in duplicate and interpolated from the Arginase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Arginase 1 concentration was determined to be 6383.5 pg/mL in 5 µg /mL liver extract.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab230930 は 3 報の論文で使用されています。
- Peng B et al. The Expansion of Myeloid-Derived Suppressor Cells Correlates With the Severity of Pneumonia in Kidney Transplant Patients. Front Med (Lausanne) 9:795392 (2022). PubMed: 35242775
- Li X et al. Elevated serum extracellular vesicle arginase 1 in type 2 diabetes mellitus: a cross-sectional study in middle-aged and elderly population. BMC Endocr Disord 22:62 (2022). PubMed: 35277141
- Tang L et al. SPLUNC1 regulates LPS-induced progression of nasopharyngeal carcinoma and proliferation of myeloid-derived suppressor cells. Med Oncol 39:214 (2022). PubMed: 36175598