Human Apolipoprotein AII ELISA Kit (APOA2) (ab108805)
Key features and details
- Sensitivity: 0.28 µg/ml
- Range: 1 µg/ml - 8 µg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Apolipoprotein AII ELISA Kit (APOA2)
Apolipoprotein A II/ApoA-II キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 4.7% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 9.9% -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.28 µg/ml -
検出範囲
1 µg/ml - 8 µg/ml -
添加回収試験
97 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Apolipoprotein AII (APOA2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AII concentrations in plasma, serum, cell culture, cell lysates and tissue samples.
An Apolipoprotein AII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AII captured in plate.
Get better reproducibility in only 90 minutes with Human Apolipoprotein AII ELISA Kit (ab184859) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 30X Biotinylated Human Apolipoprotein AII Antibody 1 x 120µl Apolipoprotein AII Microplate (12 x 8 well strips) 1 unit Apolipoprotein AII Standard 2 vials Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
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機能
May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. -
組織特異性
Plasma; synthesized in the liver and intestine. -
配列類似性
Belongs to the apolipoprotein A2 family. -
翻訳後修飾
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted. - Information by UniProt
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別名
- APO A2
- Apo AII
- Apo-AII
see all -
参照データベース
- Entrez Gene: 336 Human
- Omim: 107670 Human
- SwissProt: P02652 Human
- Unigene: 237658 Human
関連製品
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SimpleStep ELISA kits
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108805 は 1 報の論文で使用されています。
- Niu R et al. iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis. PLoS One 12:e0170741 (2017). ELISA ; Human . PubMed: 28122020