Human Apolipoprotein AI ELISA Kit (APOA1) (ab108804)

Only the assays for APOB (ab108807) and APOE (ab108813) have been tested with CSF. They are suitable for this kind of sample. The APOA1 assays have not been tested with CSF yet, but there are plans to test ab108803. Kit ab108804, the competitive ELISA for APOA1, will not be tested with CSF, and it is not known if it is suitable for assays of CSF.

Vielen Dank für Ihre Anfrage.


Leider haben wir kein weiteres Kit der Charge GR104136 mehr in unserem Lager (weltweit).

Diese Kits ist normalerweise sehr zuverlässig, aber Sie haben Recht, es gibt eine Komponente, die nie gleich ist: Der Standart in ist ein aufgereinigtes humanes Protein, das in keinem Lot die gleiche Konzentration aufweist. Das heißt, für jedes Lot muss eine neue Etablierung stattfinden, und daher kann es zu so starken Abweichungen zwischen den verschieden Lots kommen.


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2) Apolipoprotein E Human ELISA Kit
3) Haptoglobin Human ELISA Kit
4) Hemopexin Human ELISA Kit
5) Kininogen Human ELISA Kit
6) Retinol binding Protein 4 Human ELISA Kit

I'm sorry that this information could not be more helpful. Please contact us with any other questions.

Below is the information for the standard specific to your lot:

Standard Curve: Reconstitute the 120 mg of Apo A-I Standard with 3 ml of Diluent to generate a solution of 40 mg/ml. Allow the standard to sit for 10 minutes with gentle agitation prior to making dilutions. Prepare duplicate or triplicate standard points by serially diluting the standard solution (40 mg/ml) 1:2 with equal volume Diluent to produce 20, 10, 5, 2.5, 1.25, and 0.625 mg/ml solutions. Diluent serves as the zero standard (0 mg/ml). Any remaining solution should be frozen at -200C.

I hope this information is helpful to you. Please do not hesitate to contact us with any further questions.

I contacted the laboratory, and they responded that the Apo A-1 standard should be in micrograms, not nanograms. The current lot's standard is delivered at 60ug and 3mL of diluent would make the 20ug/ml solution. If you respond with your specific lot# or order# I can tell you what the standard size for your lot should have been.

Vielen Dank für Ihre Anfrage. Ich entschuldige mich für die Wartezeit, ich musste auf nähere Angaben aus dem produzierenden Labor warten.

Ich freue mich Ihnen nun bestätigen zu können, dass beide Standards aus aufgereinigten humanen Proteinen bestehen.

Die Menge an Standard ist Lot-abhängig und kann auf dem Röhrchen gefunden werden.

Ich wünsche Ihnen viel Erfolg für Ihre Versuche und verbleibe

Thank you for contacting Abcam.

After receiving the customer's complaint and abreview showing cross-reactivity with apoB using the kit ab108804 in May, we retested the kit for cross-reactivity. There was no significant cross-reactivity with other Apo proteins in the retesting. We used pure proteins and tested cross-reactivity at high concentration (same concentration as one of the highest standard point). It was suspected that the customer may not have used pure apoB protein, potentially explaining the results.

The cross-reactivity testing results are given below:




Species
% Cross Reactivity

Canine
1%

Bovine
None

Monkey
<15% (Suggest dilution 1:20 for plasma/serum)

Mouse
1%

Rat
1%

Swine
1%

Rabbit
None

Proteins
% Cross Reactivity

Apo AII
<5%

Apo B
<5%

Apo CI
<5%

Apo CII
<5%

Apo CIII
<5%

Apo E
<5%

Apo H
<5%

Apo M
<5%

Apo AI
100%






I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Here is the shelf life information you requested:
ab108804 - 6 months if kit is unused and reagents stored at 4 deg C or -20 deg C as appropriate; 1 month after opening and limiting reagent is microtiter plate storage
ab108807 - 6 months if kit is unused and reagents stored at 4 deg C or -20 deg C as appropriate; 1 month after opening and limiting reagents are microtiter plate and opened Diluent storage
ab108813- 6 months if kit is unused and reagents stored at 4 deg C or -20 deg C as appropriate; 1 month after opening and limiting reagents are microtiter plate and opened Diluent storage