Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Human Apolipoprotein AI ELISA Kit (APOA1) (ab108803)

Reviews (1)Q&A (5)References (3)
Sandwich ELISA - ab108803 Apolipoprotein AI (APOA1) Human ELISA Kit
  • Typical Standard Curve

Key features and details

  • Sensitivity: 3 ng/ml
  • Range: 3.125 ng/ml - 200 ng/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human Apolipoprotein AI ELISA Kit (APOA1)
    Apolipoprotein A I キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Overall 4.3%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Overall 9.7%

  • サンプルの種類

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Cerebral Spinal Fluid

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    = 3 ng/ml

  • 検出範囲

    3.125 ng/ml - 200 ng/ml

  • 添加回収試験

    97 %

  • 全工程の試験時間

    4h 00m

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Abcam’s Apolipoprotein AI (APOA1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AI concentrations in plasma, serum, urine, saliva, milk, CSF and cell culture samples.


    An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AI captured in plate.


    Get results in 90 minutes with Human Apolipoprotein AI ELISA Kit (ab189576) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • 試験プラットフォーム

    Microplate

製品の特性

関連製品

画像

  • Sandwich ELISA - ab108803 Apolipoprotein AI (APOA1) Human ELISA Kit
    Sandwich ELISA - ab108803 Apolipoprotein AI (APOA1) Human ELISA Kit

    APOA1 measured in various samples showing quantity (microgram) per mL of tested sample.

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108803

参考文献 (3)

ab108803 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108803 は 3 報の論文で使用されています。

  • Shearn AIU  et al. Analysis of Neat Biofluids Obtained During Cardiac Surgery Using Nanoparticle Tracking Analysis: Methodological Considerations. Front Cell Dev Biol 8:367 (2020). PubMed: 32528952
  • Huang LH  et al. Interleukin-17 Drives Interstitial Entrapment of Tissue Lipoproteins in Experimental Psoriasis. Cell Metab 29:475-487.e7 (2019). PubMed: 30415924
  • Marsche G  et al. Adiponectin Predicts High-Density Lipoprotein Cholesterol Efflux Capacity in Adults Irrespective of Body Mass Index and Fat Distribution. J Clin Endocrinol Metab 102:4117-4123 (2017). PubMed: 28938424

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Measure APOA1 in plasma samples

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
I measured APOA1 in plasma sample using ab108803 which should measure in cell culture supernatant, saliva, milk, urine, or cerebral spinal fluid. I tried to find the dilution factor for plasma samples. I found that 1:50000 is the optimum ratio for dilution and detection. Ultimately, this product can measure APOA1 in plasma samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 28 2016

Question

amount of standard for lot GR132237-1

Read More
Answer


The amount of standard for lot GR132237-1 = 3.2 ug (micrograms).

Read More

Question

can kit work with bovine samples?

Read More
Answer

Thank you for contacting us.

The lab let me know that the kit did not work for Bovine samples.

I am sorry for the negative information, but I hope it is stillhelpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Hi I am thinking of buying ELISA kits to measure apolipoprotein E and A, 5 kits of each cat number Ab 108813 and cat number Ab108804 or ab108803.

Please could you give me a quote for 10 kits, 5 of each.

Please can you explain the difference between the Apo A kits 108804 and 108803, is it just the sensitivity and which would you recommend for serum samples, bearing in mind we have a very small amount of sample.
Please could you confirm whether the diluent for diluting the sample is the same in all the kits.
Thak very much.

Read More
Answer

Thank you for your message.

I can confirm that the main difference between these kits is that the ab108804 Apolipoprotein A1 Human ELISA Kit uses a competitive ELISA quantification, whereas ab108803 Apolipoprotein A I Human ELISA Kituses standard sandwhich ELISA. Details are provided in the protocols, which are linked from the datasheets:
ab108803: https://www.abcam.com/ps/products/108/ab108803/documents/ab108803%20Apolipoprotein%20A%20I%20Human%20ELISA%20Kit%20(Website).pdf

ab108804: https://www.abcam.com/ps/products/108/ab108804/documents/ab108804%20Apolipoprotein%20A1%20Human%20ELISA%20Kit%20(Website).pdf

Also, these kits are designed for use on different samples types, the details of which are on the datasheets. ab108804 is for Serum and Plasma samples. ab108803 is for Cell culture supernatant, Saliva, Milk, Urine, Cell culture extracts.

I hope this will be helpful to you. I am forwarding your enquiry back to our marketing department who will be able to help answer your questions regarding bulk ordering.

If you have any further technical questions, please do not hesitate to contact our scientific support team at mailto:technical@abcam.com.

Read More

Question

Thank you for the help.Here comes more detailed information about what has been done when using the kit.

Diluent

The concentrated diluent was diluted 1:10 (1.2 ml to 10.8 ml water)with reagent grade water.

Standard curve
4ml of diluentwas added to the lyophilized Apo A-I.It was stated on thevial that it contained 3.2 ug protein. The stock solution therefore should contain 800 ng/ml. This stock solution was then diluted 1:4 (100 ul to 300 ul diluent) resulting in a concentration of 200 ng/ml, which was used as the first point on the standard curve. Then serial dilutions (1:2, 200 ul to 200 diluent) was performed to obtain the remaining points (3.13, 6.25, 12.5, 25, 50, 100ng/ml)on the curve. 50 ul ofstandard was added tothe well.

Biotinylated Human Apolipoprotein A-I Antibody:
18 ul ofBiotinylated Human Apolipoprotein A-I Antibodysolution was added to1782 ul diluent. 50 ul of this diluted BiotinylatedApo A-I Antibodysolution was added to the wells.

SP conjugate:
18 ul ofSP conjugatewas added to1782 ul diluent. 50 ul of this dilutedSP conjugatewas added to the wells.



What was done:

1. Standards and samples were added (50ul)to the wells andincubated for 2 hours at room temperature (20-22C).

2. The plate was washed five times.

3. Biotinylated Human Apo A-I Antibody was added and the plate was incubated for 1 hour at room temperature.

4. The plate was washed five times.

5.SP conjugatewas added and the plate was incubated for30 minat room temperature.

6. The plate was washed five times.

7. 50 ul Chromogen Substrate was added and the plate was incubated for 20 min (20 min the first time the kit was used and 30 min the second time) at room temperature.

8. 50 ul of Stop Solution was added and absorbance was read at450 nm.


Best Regards,

Read More
Answer

Thank you for providing this further information.

I have also forwarded this information to the laboratory in order to investigate the problem together. The laboratory and I do agree that all steps have been performed as indicated and do not know where the origin of the problem is. We did not get anyother negative feedback on this kit neither. We therefore conclude that some component in the kit has got damaged either during transport or storage. I would be happy to send you a free of charge replacement kit of a new batch. Please let me know whether you accept the free of charge replacement kit or if you prefer the kit to be credited/reimbursed.

I apologize for this inconvenience. Please let me know if you have any questions or concerns.

I am looking forward to hear back from you.

Read More

Question

Hello,

I am trying to determine the concentration ofApolipoprotein A-Iin nasal lavage samples using theApolipoprotein A-I HumanELISA Kit (ab108803) from abcam. My problem is that I get verylow absorbance valuesfor the standardsamples (and my own samples). The first time I tried itI got absorbance values around0.6 forthe standardsamples of 200 ng/ml and 0.1 for blank samples. I could not see any difference in absorbance values for the lowest points (0-12.5). That time I waited for 20 min until I added the Stop solution. The second time I tried it I waited for30 min and I got absorbance values of 0.5 for the 200 ng/ml samples and 0.07 for the blank samples (and the lowest point samples).Now I only have ELISA material for one more try. What would be the best thing to do at this point? Should Iincrease the incubation temperature at some step?All steps have been conducted at room temperature. Should I increase some incubations times or other factors in the protocol (Iamclosely following the protocolfor this kit)?



Best Regards,

Read More
Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I have contacted the laboratory to find out whether there might has been a problem with this lot. However we are not aware of any problem. Please find here also the sample standard data for the lot xx .




ng/ml



A
200
2.025
2.074

B
100
1.35
1.424

C
50
0.808
0.693

D
25
0.299
0.368

E
12.5
0.183
0.178

F
6.25
0.114
0.098

G
3.13
0.076
0.071

H
0
0.013
0.011






In order for us to be able to investigate this issue further, can you please write all the details of the reagent preparation? (e.g. How has the standard been reconstituted and how were the standard point diluted?)

If we conclude that the kit you received was faulty, we would be happy to replace or refund the kit.
Thank you very much for your cooperation. I am looking forward to hear back from you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com