Human Apolipoprotein A-IV ELISA Kit (ab214567)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.4 pg/ml
- Range: 250 pg/ml - 16000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Apolipoprotein A-IV ELISA Kit
APOA4/Apo-AIV キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 3.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 6.4% -
サンプルの種類
Cell culture supernatant, Saliva, Urine, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
5.4 pg/ml -
検出範囲
250 pg/ml - 16000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Saliva 78 76% - 79% Urine 97 95% - 98% Serum 98 90% - 101% Tissue Extracts 103 102% - 105% Cell culture media 103 102% - 103% Hep Plasma 98 95% - 101% EDTA Plasma 104 102% - 107% Cit plasma 91 90% - 94% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human
非交差種: Cow -
製品の概要
Human Apolipoprotein A-IV ELISA Kit (ab214567) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apolipoprotein A-IV protein in cit plasma, edta plasma, hep plasma, saliva, serum, tissue extracts, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Apolipoprotein A-IV with 5.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Human Apolipoprotein A-IV is a 45kDa glycoprotein with activities involving lipid transport. Specifically, Apolipoprotein A-IV is a component of high density lipoprotein (HDL) and chylomicrons. Its primarily synthesized is the intestine in association with lymph chylomicron particles. Although its precise function is not known, Apolipoprotein A-IV is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. Human Apolipoprotein A-IV consists of a 20 amino acid (aa) signal peptide and 376 aa mature chain. Human Apolipoprotein A-IV is 62% identical to both mouse and rat Apolipoprotein A-IV.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml 10X Human Apolipoprotein A-IV Capture Antibody 1 x 600µl 10X Human Apolipoprotein A-IV Detector Antibody 1 x 600µl Human Apolipoprotein A-IV Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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関連性
APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties -
細胞内局在
Secreted -
別名
- Apo AIV
- APOA 4
- ApoA IV
see all -
参照データベース
- Entrez Gene: 337 Human
- Omim: 107690 Human
- SwissProt: P06727 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:32,000, plasma (citrate) 1:16,000, plasma (EDTA) 1:16,000, and plasma (heparin) 1:16000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 71.65 µg/mL in neat serum, 48.04 µg/mL in neat plasma (citrate), 42.94 µg/mL in neat plasma (EDTA) and 51.18 µg/mL in neat plasma (heparin).
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The concentrations of Apolipoprotein A-IV were measured in duplicate and interpolated from the Apolipoprotein A-IV standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 4,829 pg/mL in placenta extract and 17,016 pg/mL in liver homogenate extract.
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The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 6.25% and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 177,941 pg/mL in neat saliva (spiked) and 80,680 pg/mL in neat urine (spiked).
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Saliva was analyzed at 6.25% and urine at 12.5%. The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 25,370 pg/mL in neat saliva (native) and 3903 pg/mL in neat urine (native).
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean human Apolipoprotein A-IV concentration was determined to be 192.8 µg/mL with a range of 9.2 – 418.0 µg/mL.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab214567 は 1 報の論文で使用されています。
- Zeng J et al. Increased serum protein levels by Yuanshi Shengmai Chenggu Tablet in treatment of avascular osteonecrosis of the femoral head. Mol Med Rep 17:2121-2126 (2018). PubMed: 29207081