製品の概要

  • 製品名

    Human Antithrombin III ELISA Kit
    Antithrombin III キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    1 20 3.1%
    2 20 3.2%
    3 20 3.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    1 20 8.9%
    2 20 9%
    3 20 9.1%
  • サンプルの種類

    Serum, Plasma
  • アッセイタイプ

    Competitive
  • 検出感度

    = 0.03 µg/ml
  • 検出範囲

    0.063 µg/ml - 4 µg/ml
  • 添加回収試験

    96.5 %

  • 全工程の試験時間

    2h 40m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Human
  • 製品の概要

    The Human Antithrombin III ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab220073) is designed for detection of Human Antithrombin III (AT3) in plasma and serum samples.


    This assay employs a quantitative competitive enzyme immunoassay technique that measures Antithrombin III in less than 3 hours. A polyclonal antibody specific for Antithrombin III has been pre-coated onto a 96-well microplate with removable strips. Antithrombin III in standards and samples compete with a biotinylated Antithrombin III sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • 特記事項

    The serine protease inhibitor antithrombin III, the most important natural inhibitor of thrombin activity, has been shown to exert marked anti-inflammatory properties. AT3 levels are positively correlated with plasma total cholesterol levels, plasma low-density lipoprotein cholesterol levels, plasma triglycerides and D-dimer levels.

  • アプリケーション

    適用あり: Competitive ELISAmore details
  • 試験プラットフォーム

    Microplate (12 x 8 well strips)

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab220073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

画像

  • Human ATIII Standard curve (ab220073)

プロトコール

参考文献

ab220073 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab220073.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録