Human Annexin A1 抗体 Pair - BSA and Azide free (ab270365)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
製品の概要
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製品名
Human Annexin A1 Antibody Pair - BSA and Azide free
Annexin A1/ANXA1 キット 製品一覧 -
アッセイタイプ
ELISA set -
検出範囲
0.313 pg/ml - 20 pg/ml -
種交差性
交差種: Human -
製品の概要
The Antibody Pair can be used to quantify Human Annexin A1. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab267808), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
キャリア・フリー
はい -
内容 10 x 96 tests Human Annexin A1 Capture Antibody (unconjugated) 1 x 100µg Human Annexin A1 Detector Antibody (unconjugated) 1 x 100µg -
研究分野
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機能
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. -
配列類似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
ドメイン
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻訳後修飾
Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. -
細胞内局在
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages. - Information by UniProt
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別名
- Annexin 1
- Annexin A1
- Annexin I
see all -
参照データベース
- Entrez Gene: 301 Human
- Omim: 151690 Human
- SwissProt: P04083 Human
- Unigene: 494173 Human
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Sandwich ELISA - Human Annexin A1 Antibody Pair - BSA and Azide free (ab270365)To learn more about the advantages of recombinant antibodies see here.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab270365 は論文での使用が確認できていません。