Human Amphiregulin ELISA Kit (ab222504)
製品の概要
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製品名Human Amphiregulin ELISA Kit
Amphiregulin キット 製品一覧 -
検出方法Colorimetric
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再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Amphiregulin 3 1.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Amphiregulin 5 4.1% -
サンプルの種類Cell culture supernatant, Serum, EDTA Plasma, Citrate Plasma
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アッセイタイプSandwich (quantitative)
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検出感度2.4 pg/ml
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検出範囲18 pg/ml - 600 pg/ml
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添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 106 84% - 117% Cell culture media 108 106% - 110% EDTA Plasma 98 91% - 104% Citrate Plasma 103 93% - 111% -
全工程の試験時間1h 30m
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ステップOne step assay
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種交差性交差種: Human
非交差種: Mouse -
製品の概要
Amphiregulin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human Amphiregulin protein in serum, plasma (citrate and EDTA), and cell culture supernatants.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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アプリケーション適用あり: Sandwich ELISAmore details
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試験プラットフォームPre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法Store at +4°C. Please refer to protocols.
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内容 1 x 96 tests 10X Human Amphiregulin Capture Antibody 1 x 600µl 10X Human Amphiregulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI 1 x 6ml Human Amphiregulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Sample Diluent NS 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
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配列類似性Belongs to the amphiregulin family.
Contains 1 EGF-like domain. -
細胞内局在Membrane.
- Information by UniProt
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別名
- A REG
- Amphiregulin
- Amphiregulin B
see all -
参照データベース
- Entrez Gene: 374 Human
- Entrez Gene: 727738 Human
- Omim: 104640 Human
- SwissProt: P15514 Human
- Unigene: 270833 Human
- Unigene: 645475 Human
アプリケーション
Our Abpromise guarantee covers the use of ab222504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
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Example of human Amphiregulin standard curve in Sample Diluent NS.The Amphiregulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked Amphiregulin in human serum, plasma and cell culture supernatant samples.The concentrations of Amphiregulin were measured in duplicates, interpolated from the Amphiregulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (citrate) 50%, RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native Amphiregulin in human MCF7 cell culture supernatant.Human MCF7s were stimulated with 2% PHA for 3 days before the supernatant was collected and assayed. The concentrations of Amphiregulin were measured in duplicate and interpolated from the Amphiregulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Amphiregulin concentration was determined to be 23.7 ng/mL in stimulated and 16 ng/mL in unstimulated MCF7 cell culture supernatant.
プロトコール
参考文献
ab222504 has not yet been referenced specifically in any publications.
