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Human alpha Fetoprotein ELISA Kit (ab108631)

Submit a review Q&A (1)References (1)
Typical Standard Curve

    Key features and details

    • Sensitivity: 2 ng/ml
    • Range: 2 ng/ml - 300 ng/ml
    • Sample type: Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    SimpleStep ELISAキットで、高再現性を実現

    Product image
    Human alpha Fetoprotein ELISA Kit (ab193765)
    • 洗浄 1 回、アッセイ時間は 90 分
    • 高品質の抗体を採用し、高い再現性と感度を実現
    • すべてのキットが生体サンプルで評価済み
    • 96ウェルプレートは、12 x 8に分割可能

    製品の概要

    • 製品名

      Human alpha Fetoprotein ELISA Kit
      alpha 1 Fetoprotein キット 製品一覧

    • 検出方法

      Colorimetric

    • サンプルの種類

      Serum

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      = 2 ng/ml

    • 検出範囲

      2 ng/ml - 300 ng/ml

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s alpha Fetoprotein (AFP) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in Human serum.

      A 96-well plate has been precoated with anti-alpha Fetoprotein antibodies. Samples and standards and are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-alpha Fetoprotein-HRP conjugate binds to the antibody-alpha Fetoprotein complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.

    • 試験プラットフォーム

      Microplate

    製品の特性

    • 保存方法

      Store at +4°C. Please refer to protocols.
    • 内容 1 x 96 tests
      alpha Fetoprotein Standard - 0 ng/mL 1 x 0.5ml
      alpha Fetoprotein Standard - 150 ng/mL 1 x 0.5ml
      alpha Fetoprotein Standard - 20 ng/mL 1 x 0.5ml
      alpha Fetoprotein Standard - 300 ng/mL 1 x 0.5ml
      alpha Fetoprotein Standard - 5 ng/mL 1 x 0.5ml
      alpha Fetoprotein Standard - 50 ng/mL 1 x 0.5ml
      Anti-alpha Fetoprotein Coated Microplate (12 x 8 wells) 1 unit
      Anti-alpha Fetoprotein HRP Conjugate 1 x 18ml
      Stop Solution 1 x 11ml
      TMB Reagent 1 x 11ml
      Zero Buffer 1 x 13ml

    • 研究分野

    • 機能

      Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

    • 組織特異性

      Plasma. Synthesized by the fetal liver and yolk sac.

    • 配列類似性

      Belongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.

    • 発生段階

      Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

    • 翻訳後修飾

      Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
      Sulfated.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 別名

      • Afp
      • AFPD
      • Alpha fetoglobulin
      • Alpha fetoprotein
      • Alpha fetoprotein precursor
      • Alpha-1-fetoprotein
      • Alpha-fetoglobulin
      • Alpha-fetoprotein
      • alpha-fetoprotein, Hereditary persistence of, included
      • FETA
      • FETA_HUMAN
      • Hereditary persistence of alpha fetoprotein
      • HPAFP
      see all

    • 参照データベース

      画像

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab108631

      参考文献 (1)

      ab108631 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab108631 は 1 報の論文で使用されています。

      • Lv S  et al. H2-Based Electrochemical Biosensor with Pd Nanowires@ZIF-67 Molecular Sieve Bilayered Sensing Interface for Immunoassay. Anal Chem 91:12055-12062 (2019). PubMed: 31436433

      レビューと Q&A

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      Question

      Dear technical team, Our customer would like to purchase CEA ELISA kit and alpha Fetoprotein ELISA Kit. She has searched ab108631, ab108838, ab108635, ab99992 and asked well plate type.   Is it strip type or one plate type?  

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      Answer

      Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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