Human alpha 2 Macroglobulin ELISA Kit (ab108888)

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製品の概要

  • 製品名

    Human alpha 2 Macroglobulin ELISA Kit
    alpha 2 Macroglobulin キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Overall 5.1%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Overall 9.8%

  • サンプルの種類

    Serum, Plasma

  • アッセイタイプ

    Competitive

  • 検出感度

    = 0.95 µg/ml

  • 検出範囲

    1.25 µg/ml - 40 µg/ml

  • 添加回収試験

    98 %

  • 全工程の試験時間

    3h 00m

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Abcam’s alpha 2 Macroglobulin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in plasma and serum.


    An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 2 Macroglobulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 2 Macroglobulin captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Competitive ELISAmore details

  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent dilution.

画像

  • Competitive ELISA - alpha 2 Macroglobulin Human ELISA Kit (ab108888)
    Competitive ELISA - alpha 2 Macroglobulin Human ELISA Kit (ab108888)
    Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Competitive ELISA - alpha 2 Macroglobulin Human ELISA Kit (ab108888)
    Competitive ELISA - alpha 2 Macroglobulin Human ELISA Kit (ab108888)

    Alpha 2 Macroglobulin measured in biological fluids and cell culture supernatant showing quantity (ug) per mL of tested sample. Human serum diluted 400-1600 fold. Human plasma diluted 80-320 fold. Other samples undiluted.

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参考文献

This product has been referenced in:

  • Ghale-Noie ZN  et al. High Serum Alpha-2-Macroglobulin Level in Patients with Osteonecrosis of the Femoral Head. Arch Bone Jt Surg 6:219-224 (2018). Read more (PubMed: 29911139) »
  • Bjelosevic S  et al. Quantitative Age-specific Variability of Plasma Proteins in Healthy Neonates, Children and Adults. Mol Cell Proteomics 16:924-935 (2017). Read more (PubMed: 28336724) »
See all 4 Publications for this product

ab108888 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Question

Kindly contacted us to discuss sample collection.

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Answer

The lab has informed me that your sample collection method is fine and that the anti-coagulant will not affect the assay. You can include the anticoagulant.

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Question

for the preparation of plasma samples, We use a citrate solution that is not exactly a sodium citrate 0.1M as specified in the protocol. Did you test other methods/solutions that did not work with this kit? Do you think my samples will work? Thank you!

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Answer

Thank you for contacting us.

The lab said the other option besides sodium citrate 0.1M is the ready use tube that clinical lab used to draw
human plasma for your samples.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I will be using samples that are pre-anticoagulated (states that the anticoagulation is performed with ATC). The protocol states that sodium citrate, EDTA or heparin can be used with the kit. Will there be an issue with using these samples with the kit? Should we perform another anticoagulation step with one of the above?

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Answer

Thank you for contacting us.

Unfortunately the laboratory has no data regarding on ATC samples, and cannot give a recommendation here.

Please do not hesitate to contact us if you need any more advice or information.

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Question

What volume should the standard be reconstituted with? Lot # GR100954-1

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Answer

Thank you very much for your call yesterday and for your patience while I've been in touch with my colleagues regarding this standard reconstitution volume.

For this lot, the standard vial contains 160 ug of human alpha-2-macroglobulin. So, to generate a stock solution of 40 ug/mL, you will need to reconstitute the standard in 4 mL of 1x diluent.

I hope this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help.

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