Human alpha 2 Macroglobulin ELISA Kit (ab108888)
Key features and details
- Sensitivity: 0.95 µg/ml
- Range: 1.25 µg/ml - 40 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Human alpha 2 Macroglobulin ELISA Kit
alpha 2 Macroglobulin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.7% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.6% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Competitive -
検出感度
= 0.95 µg/ml -
検出範囲
1.25 µg/ml - 40 µg/ml -
添加回収試験
98 %
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全工程の試験時間
3h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s alpha 2 Macroglobulin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in plasma and serum.
An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 2 Macroglobulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 2 Macroglobulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 3X Biotinylated alpha 2 Macroglobulin (Lyophilized) 1 vial alpha 2 Macroglobulin Microplate (12 x 8 well strips) 1 unit alpha 2 Macroglobulin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. -
組織特異性
Secreted in plasma. -
配列類似性
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
発生段階
Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. -
細胞内局在
Secreted. - Information by UniProt
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別名
- A2m
- A2MG_HUMAN
- Alpha 2 M
see all -
参照データベース
- Entrez Gene: 2 Human
- Omim: 103950 Human
- SwissProt: P01023 Human
- Unigene: 212838 Human
画像
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Competitive ELISA - alpha 2 Macroglobulin Human ELISA Kit (ab108888)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Competitive ELISA - alpha 2 Macroglobulin Human ELISA Kit (ab108888)Alpha 2 Macroglobulin measured in biological fluids and cell culture supernatant showing quantity (ug) per mL of tested sample. Human serum diluted 400-1600 fold. Human plasma diluted 80-320 fold. Other samples undiluted.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab108888 は 5 報の論文で使用されています。
- Gupta AK et al. Cerebrospinal Fluid Proteomics For Identification Of a2-Macroglobulin As A Potential Biomarker To Monitor Pharmacological Therapeutic Efficacy In Dopamine Dictated Disease States Of Parkinson's Disease And Schizophrenia. Neuropsychiatr Dis Treat 15:2853-2867 (2019). PubMed: 31632033
- Ghale-Noie ZN et al. High Serum Alpha-2-Macroglobulin Level in Patients with Osteonecrosis of the Femoral Head. Arch Bone Jt Surg 6:219-224 (2018). PubMed: 29911139
- Bjelosevic S et al. Quantitative Age-specific Variability of Plasma Proteins in Healthy Neonates, Children and Adults. Mol Cell Proteomics 16:924-935 (2017). PubMed: 28336724
- Robinson MD et al. Water T2as an early, global and practical biomarker for metabolic syndrome: an observational cross-sectional study. J Transl Med 15:258 (2017). PubMed: 29258604
- Sunderic M et al. Protein molecular forms of insulin-like growth factor binding protein-2 change with aging. Exp Gerontol 58C:154-158 (2014). PubMed: 25106097