製品の概要

  • 製品名

    Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)
    alpha 1 Antitrypsin キット 製品一覧
  • 検出方法

    Colorimetric
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3.7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8.6%
  • サンプルの種類

    Serum, Plasma
  • アッセイタイプ

    Competitive
  • 検出感度

    = 0.018 µg/ml
  • 検出範囲

    0.039 µg/ml - 40 µg/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間

    3h 00m
  • ステップ

    Multiple steps standard assay
  • 種交差性

    交差種: Human
  • 製品の概要

    alpha 1 Antitrypsin (SERPINA1) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108798) is designed for the quantitative measurement of alpha 1 antitrypsin levels in plasma and serum.


    An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 1 Antitrypsin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 1 antitrypsin captured in plate.


    Get results in 90 minutes with Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • アプリケーション

    適用あり: Competitive ELISAmore details
  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 1 x 30ml
    2X Biotinylated Human alpha 1 Antitrypsin (Lyophilized) 1 vial
    alpha 1 Antitrypsin Microplate (12 x 8 well strips) 1 unit
    alpha 1 Antitrypsin Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野

  • 機能

    Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • 組織特異性

    Ubiquitous. Expressed in leukocytes and plasma.
  • 関連疾患

    Alpha-1-antitrypsin deficiency
  • 配列類似性

    Belongs to the serpin family.
  • ドメイン

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • 翻訳後修飾

    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • 細胞内局在

    Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 別名

    • A1A
    • A1AT
    • A1AT_HUMAN
    • AAT
    • Alpha 1 antiproteinase
    • Alpha 1 antitrypsin
    • Alpha 1 antitrypsin null
    • Alpha 1 protease inhibitor
    • Alpha-1 protease inhibitor
    • Alpha-1-antiproteinase
    • alpha1 proteinase inhibitor
    • Alpha1AT
    • Dom1
    • PI
    • PI1
    • PRO0684
    • PRO2275
    • Serine (or cysteine) proteinase inhibitor clade A member 1
    • Serine protease inhibitor 1-1
    • Serine protease inhibitor A1a
    • Serpin A1
    • Serpin A1a
    • Serpin peptidase inhibitor clade A member 1
    • Serpina1
    • Short peptide from AAT
    • SPAAT
    • Spi1-1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

画像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Alpha 1 Antitrypsin measured in biological fluids showing quantity (μg) per mL of tested sample. Serum and plasma samples diluted 100-8100 fold. Human milk and saliva diluted 10 fold. Hu urine diluted 1000 fold.

プロトコール

参考文献

This product has been referenced in:

  • Abdullah MI  et al. Tissue and serum samples of patients with papillary thyroid cancer with and without benign background demonstrate different altered expression of proteins. PeerJ 4:e2450 (2016). Competitive ELISA ; Human . Read more (PubMed: 27672505) »
See 1 Publication for this product

レビューと Q&A

1-7 of 7 Abreviews or Q&A

Question
Answer

Thank you for contacting us.




ab108798 is for plasma and serum samples.

ab108799 is for urine, milk, saliva and cell culture samples

Unfortunately, neither of them has cross-reactivity with mouse

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

You can use ab108799 for serum, you will just have to optimize the dilutions to get on the standard curve. Our serum samples were at 6000X.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

We unfortunately have not tested these products with sputum samples. Theoretically, these products will be OK however we cannot guarantee as we do not have tested data. The best way for using these products is by including serum or plasma samples as positive control.

The ab108798 will be able detect the protein in a range between 0.039 µg/ml - 40 µg/ml and ab108799 in between 0.39 ng/ml - 100 ng/ml.

We unfortunately are not aware any citation of these products in publications. These products are guaranteed for plasma and serum samples. In case they fail we will provide you full refund. Please check the Abpromise guarantee on our website;

https://www.abcam.com/index.html?pageconfig=abpromise

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your email.

I have added now a free unit of this kit ab108798 to your next order with the reference number xx. It will be dispatched tomorrow.

I can also confirm, that I have been in correspondence with the laboratory, the only possible explanation we can have for the results is that the biotin conjugated ATT has been used instead of the standard.

We do not know at what point these two vials might have been inversed. Just in case to avoid a repeat problem, please remind the customer to be extra careful in this regards and to use the standard vial for making the standard.

Please provide again all my apologies to the customer for the misunderstandings in the communication as well. I hope the kit will work as expected for the customer and would like to wish them all the best for their research.

Thank you for your cooperation. Please do not hesitate to contact us again should you have any other questions or concerns.

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Answer

This is to confirm that we are liaising with the lab to get this specific kit component ready for shipment as soon as possible. Thank you for your patience in advance!

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Answer

Thank you for your reply.

Please could you confirm the following:

1. Did the customer centrifuge the component?
2. How much did they receive?

Looking forward to your reply.

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Answer

Thank you for your enquiry regarding ab108798.

Could you please confirm and explain why the streptavidin conjugate reagent is insufficient for 96 wells plate? Is there any shortage in the volume provided or the reagent does not work properly?

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

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