Human Alpha 1 acid Glycoprotein / AGP ELISA Kit (AGP) (ab108854)

Thank you for your inquiry.

Unfortunately, we do not have any Human serum available in our catalog that could be used as control and the kit ab108854 (https://www.abcam.com/ab108854) comes with Human AGP Standard but not with control serum.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

Thank you for your reply.

We will update the graph, as it indeed confusing. When +2, 1, 0, -1 and -2is written, it means actually 102, 101, 100, 10-1, 10-2 so 100, 10, 1, 0.1 and 0.01.

Please excuse for this error. As said, I already sent the request to update the graph. Please let me know if you have any other concern or questions.

I wish you a great weekend!

Thank you for contacting us and your patience while we were investigating this issue.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. We do also guarantee all our products to work as stated on the datasheet.

I would be pleased to look into your results with more details to provide more specific answers and to help you to solve the problem. Can you therefore please send me the raw annotated data?

Please find here also the answers to your questions:

1.) The standard curve example on the datasheet is plotted in log, that is why the it seems that it goes not to 4ug/ml, but actually it does. I have requested now that this should be annotated more clearly on the datasheet. Thank you for pointing this out to us.
The laboratory also sent us an example of the raw values obtained, please find them here:

STD ug/ml Std avg OD

4 0.106

2 0.226

1 0.367

0.5 0.699

0.25 1.097

0.125 1.317

0.063 1.563

0 1.806



2.) I am happy also to confirm that this is a competition ELISA assay. The protein from the standards and samples is put into the wells at the same time as the biotinylated protein from the kit. This forces the two to compete for the binding sites of the antibody on the plate. However the fluorescing reagents will only bind to the biotin on the kit protein. So the less protein in the standard and sample, the less chance it has of outcompeting the kit protein. This does result in an inverse relationship between the vivacity of the color and the amount of AGP from the standard and samples.

3.) Yes the dilution should be 1000x. We recommend doing a serial dilution to ensure thorough mixing and accurate results. First do 100x then a further 10x.
The laboratory obtained the following results typically with serum or plasma:

Plasma 1000x avg OD 0.359

Serum 1000x avg OD 0.193

I hope this information is helpful. Please do not hesitate to send us over more information, so we cantry to help furtherto analyze the origin of the problem. We do guarantee all our products to work as stated on the datasheet, should therefore be a problem with the product, we would be happy to replace it.

I look forward to receiving your reply. Please let me know also if you have any further questions or concerns.

(I am currently also searchingfor your other question in regardsto theserum dilutions of the other kits- I will get back to you as soon as Iknow more).

Thank you for your inquiry.

I can recommend ab108854 for your customer since this kit is tested and guaranteed for plasma and serum samples. ab108852 is not.

Please find the protocol booklet for ab108854 attached.

Plasma and serum samples can usually be stored at -20C for long term storage. These samplesneed to be fully thawed before using them with the kit ab108854.

I hope this in formation is helpful for your customer and wish them good luck with their research.