Human Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AGP) (ab108852)
Key features and details
- Sensitivity: 2.3 ng/ml
- Range: 12.5 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human Alpha 1 Acid Glycoprotein / AGP ELISA Kit (AGP)
Alpha 1 Acid Glycoprotein/AGP キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.8% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 2.3 ng/ml -
検出範囲
12.5 ng/ml - 100 ng/ml -
添加回収試験
= 96 %
-
全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Alpha 1 Acid Glycoprotein / AGP Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of AGP levels in cell culture supernatant, saliva, milk, and urine.
An Alpha 1 Acid Glycoprotein / AGP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Alpha 1 Acid Glycoprotein / AGP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Alpha 1 Acid Glycoprotein / AGP captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Alpha 1 Acid Glycoprotein / AGP Antibody 1 x 120µl Alpha 1 Acid Glycoprotein / AGP Microplate (12 x 8 well strips) 1 unit Alpha 1 Acid Glycoprotein / AGP Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
研究分野
-
機能
Appears to function in modulating the activity of the immune system during the acute-phase reaction. -
組織特異性
Expressed by the liver and secreted in plasma. -
配列類似性
Belongs to the calycin superfamily. Lipocalin family. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- A1AG1_HUMAN
- AGP
- AGP 1
see all -
参照データベース
- Entrez Gene: 5004 Human
- Omim: 138600 Human
- SwissProt: P02763 Human
- Unigene: 522356 Human
画像
-
Typical Standard Curve
Representative Standard Curve using ab108852.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab108852 は 4 報の論文で使用されています。
- Qaisar R et al. Circulating Biomarkers of Handgrip Strength and Lung Function in Chronic Obstructive Pulmonary Disease. Int J Chron Obstruct Pulmon Dis 15:311-321 (2020). PubMed: 32103930
- Mateos J et al. Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection. Sci Rep 10:3844 (2020). PubMed: 32123229
- Zhan Z et al. Urine a-fetoprotein and orosomucoid 1 as biomarkers of hepatitis B virus-associated hepatocellular carcinoma. Am J Physiol Gastrointest Liver Physiol 318:G305-G312 (2020). PubMed: 31736338
- Chen WQ et al. Polymorphism of ORM1 is associated with the pharmacokinetics of telmisartan. PLoS One 8:e70341 (2013). PubMed: 23940561