Human Albumin ELISA Kit (ab179887)
製品の概要
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製品名
Human Albumin ELISA Kit
Albumin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 8 2.3% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 3 8.8% -
サンプルの種類
Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
210 pg/ml -
検出範囲
0.31 ng/ml - 20 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture extracts 81 81% - 82% Cell culture media 93 93% - 94% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human, Rhesus monkey
非交差種: Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Pig -
製品の概要
Albumin (ALB) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Albumin protein in human serum and plasma.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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特記事項
Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human Albumin Capture Antibody 1 x 600µl 10X Human Albumin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CP 1 x 6ml Human Albumin Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
組織特異性
Plasma. -
関連疾患
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻訳後修飾
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
細胞内局在
Secreted. - Information by UniProt
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別名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
参照データベース
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
関連製品
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Alternative Versions
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SimpleStep ELISA kits
アプリケーション
Our Abpromise guarantee covers the use of ab179887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
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Other - Human Albumin ELISA Kit (ab179887)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Albumin standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of NHS (pooled donor) within the working range of the assay.Background subtracted data from triplicate measurements are plotted.
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Observed Albumin concentration in pooled donor normal Human serumObserved ALB concentration in pooled donor normal Human serum (n=10). Mean Human serum values fall within expected normal reference ranges (ARUP/WHO).
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Observed Albumin levels in pooled donor normal Human plasmaMean Human ALB values fall within expected normal reference ranges (WHO).
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Cross Reactivity in Different Animal SerumsAnimal serum from a variety of species were serially diluted and tested for cross reactivity using the Human Serum Albumin SimpleStep ELISA kit (ab179887). Data shows that ab179887 is >4 logs more sensitive and specific to Human serum albumin compared to other animal species.
プロトコール
参考文献
This product has been referenced in:
- Smith JW et al. Aflatoxin and viral hepatitis exposures in Guatemala: Molecular biomarkers reveal a unique profile of risk factors in a region of high liver cancer incidence. PLoS One 12:e0189255 (2017). Read more (PubMed: 29236788) »
- Dickie AP et al. The pharmacokinetics and metabolism of lumiracoxib in chimeric humanized and murinized FRG mice. Biochem Pharmacol 135:139-150 (2017). ELISA ; Human . Read more (PubMed: 28351678) »
