SimpleStep

Human Albumin ELISA Kit (ab179887)

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製品の概要

  • 製品名

    Human Albumin ELISA Kit
    Albumin キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Serum 8 5.2%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Serum 3 7.6%

  • サンプルの種類

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    0.1 ng/ml

  • 検出範囲

    0.7 ng/ml - 45 ng/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 104 104% - 105%
    Serum 100 92% - 110%
    Hep Plasma 98 89% - 105%
    EDTA Plasma 104 97% - 109%
    Cit plasma 101 100% - 103%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    As of December 12, 2019 Human Albumin ELISA kit has been re-developed with new recombinant monoclonal antibodies to provide improved performance and consistency.


    Human Albumin ELISA kit (ab179887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Albumin protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Albumin with 100 pg/mL sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

  • 特記事項

    Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

  • 試験プラットフォーム

    Microplate

製品の特性

画像

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Human Albumin standard curve comparison
    Human Albumin standard curve comparison

    Standard Curve comparison between the original human Albumin SimpleStep ELISA kit current human Albumin SimpleStep ELISA kit.

  • Example of human Albumin standard curve in Sample Diluent NS.
    Example of human Albumin standard curve in Sample Diluent NS.

    The Albumin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Albumin in human serum, plasma and cell culture supernatant samples.
    Interpolated concentrations of native Albumin in human serum, plasma and cell culture supernatant samples.

    The concentrations of Albumin were measured in duplicates, interpolated from the Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000,000, plasma (EDTA) 1:4,000,000, plasma (citrate) 1:8,000,000, and plasma (heparin) 1:8,000,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 31.5 mg/mL in serum, 75.4 mg/mL in plasma (EDTA), 35.5 mg/mL in plasma (citrate) and 41.3 mg/mL in plasma (heparin).

参考文献

ab179887 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab179887 は 4 報の論文で使用されています。

  • Kang JA  et al. Ciclopirox inhibits Hepatitis B Virus secretion by blocking capsid assembly. Nat Commun 10:2184 (2019). ELISA ; Human . PubMed: 31097716
  • Smith JW  et al. Aflatoxin and viral hepatitis exposures in Guatemala: Molecular biomarkers reveal a unique profile of risk factors in a region of high liver cancer incidence. PLoS One 12:e0189255 (2017). PubMed: 29236788
  • Dickie AP  et al. The pharmacokinetics and metabolism of lumiracoxib in chimeric humanized and murinized FRG mice. Biochem Pharmacol 135:139-150 (2017). ELISA ; Human . PubMed: 28351678
  • Peng G  et al. Immobilization of native type I collagen on polypropylene fabrics as a substrate for HepG2 cell culture. J Biomater Appl 32:93-103 (2017). ELISA ; Human . PubMed: 28504559

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Review the use of kit for CSF sample

 Average Good 4/5 (Ease of Use)
Abreviews
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The kit is easy to use. But I found that the variation between the duplicate is big for both standards and samples. I diluted the CSF samples at 3 different concentrations. It showed that 1:12000 dilution worked the best.

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投稿 Dec 20 2018

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