Human AKT3 (pS473) ELISA Kit (ab270887)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 19.6 U/ml
- Range: 46.88 U/ml - 3000 U/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human AKT3 (pS473) ELISA Kit
AKT3 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Extract 3 2.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Extract 24 3.4% -
サンプルの種類
Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
19.6 U/ml -
検出範囲
46.88 U/ml - 3000 U/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell Lysate 97 94% - 107% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human ATK (pS473) ELISA kit (ab270887) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ATK (pS473) protein in human cell extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human ATK (pS473) with 5.9 units/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
AKT3 is a member of the AKT family that includes AKT1 and AKT2. These serine/threonine-protein kinases are involved in diverse processes including glucose uptake, cell cycle progression, and apoptosis. AKT3 plays an important role in brain development and is crucial for glioma cells malignancy. Phosphorylation of Serine 473 is a known regulator of AKT’s kinase activity. The antibodies for this kit were raised against an AKT3-specific N-terminal fragment, as well as a phospho-peptide encompassing the 473-serine site, which shares high sequence identity for all three AKT members. The protein standard in this product is recombinant human AKT3 ligated with peptide containing phosphorylated Serine 416.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human AKT3 (pS473) Capture Antibody 1 x 600µl 10X Human AKT3 (pS473) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human AKT3 (pS473) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
組織特異性
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
ドメイン
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
翻訳後修飾
Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
細胞内局在
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
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別名
- Akt3
- AKT3 kinase
- AKT3_HUMAN
see all -
参照データベース
- Entrez Gene: 10000 Human
- Omim: 611223 Human
- SwissProt: Q9Y243 Human
- Unigene: 498292 Human
関連製品
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Alternative Versions
画像
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human AKT3 (pS473) standard curve in 1X Cell Extraction Buffer PTR.The AKT3 (pS473) standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native AKT3 (pS473) in human SH-SY5Y +pervanadate extract 500 µg/mL extract load.The concentrations of AKT3 (pS473) were measured in duplicate and interpolated from the AKT3 (pS473) standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AKT3 (pS473) concentration was determined to be 2,820 units/mL.
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Comparison of native AKT3 (pS473) concentration in treated versus control cell extracts.SH-SY5Y cells were treated with 10 mM pervanadate for 20 minutes, scraped and buffer exchanged into PBS + 20 mM NaF, followed by protein extraction. Mock control SH-SY5Y cells accompanied cell treatment. Concentrations of AKT3 (pS473) were measured in duplicate and interpolated against the standard curve. Results show the mean AKT3 (pS473) concentration in control cells was 30.6 units/mL and 2,795 units/mL in pervanadate treated samples.
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Sandwich ELISA - Human AKT3 (pS473) ELISA Kit (ab270887)To learn more about the advantages of recombinant antibodies see here.
参考文献 (0)
ab270887 は論文での使用が確認できていません。
