Anti-HSV1 ICP0 抗体 [5H7] (ab6513)

Reviews (1)Q&A (1)References (27)

Key features and details

  • Mouse monoclonal [5H7] to HSV1 ICP0
  • Suitable for: WB, ELISA, ICC/IF
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-HSV1 ICP0 antibody [5H7]

  • 製品の詳細

    Mouse monoclonal [5H7] to HSV1 ICP0

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ELISA, ICC/IFmore details

  • 種交差性

    交差種: Other species

  • 免疫原

    Herpes Virus

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    5H7

  • ミエローマ

    NS1/1-Ag4-1

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/8000.
ELISA 1/12800.
ICC/IF 1/25600.

ターゲット情報

  • 関連性

    Herpes simplex virus (HSV) encodes four nuclear phosphoproteins that contribute towards the full productive lytic cycle cascade in newly infected permissive cells. The IE175 (or ICP4) and IE63 (or ICP27) proteins have essential regulatory roles during the lytic cycle. A third, IE110 (or ICP0), appears to play only an accessory role in the presence of VP16, but in the absence of VP16 appears to be critical for initiation of reactivated infections.

  • 細胞内局在

    Nuclear

  • 別名

    • Alpha 0 protein antibody
    • Herpes siplex virus 1 antibody
    • HHV1 ICP0 antibody
    • HSV1 IE110 antibody
    • Human Herpesvirus 1 antibody
    • IE110 antibody
    • Immediate early nuclear phosphoprotein antibody
    • Immediate early protein IE110 antibody
    • Trans acting transcriptional protein ICP0 antibody
    • VMW110 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP0 antibody [5H7] (ab6513)
    Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP0 antibody [5H7] (ab6513)This image is courtesy of an anonymous Abreview
    ab6513 (1/25600) staining HSV1 ICP0 in human cells. Cells were PFA-fixed, permeabilized in 0.3% TritonX-100/PBS and blocked in 1% serum /30 minutes /25oC. A fluorescently labeled donkey anti-mouse antibody was used as the secondary.

    See Abreview

参考文献 (27)

ab6513 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6513 は 27 報の論文で使用されています。

  • Furey C  et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
  • Subramanian G  et al. The interferon-inducible protein TDRD7 inhibits AMP-activated protein kinase and thereby restricts autophagy-independent virus replication. J Biol Chem 295:6811-6822 (2020). PubMed: 32273341
  • Wang Z  et al. MiR-155-5p modulates HSV-1 replication via the epigenetic regulation of SRSF2 gene expression. Epigenetics 14:494-503 (2019). PubMed: 30950329
  • Li F  et al. Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early Infection. Viruses 11:N/A (2019). PubMed: 31121928
  • Yan X  et al. miR-138 expression in oral herpes simplex and its effect on ICP0. Exp Ther Med 17:388-392 (2019). PubMed: 30651809
View all Publications for this product

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Immunocytochemistry/ Immunofluorescence abreview for Anti-HSV1 ICP0 antibody [5H7]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HSV1 infected or transfected with plasmid)
Specification
HSV1 infected or transfected with plasmid
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX-100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Oct 12 2007

Question

Could you let me know if this antibody can be used to stain fixed tissue sections. If not, do you have any antibodies available to HSV1 ICP0 or ICP5 that can be used to immunohistochemically stain slides?

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Answer

According to the datasheet, this antibody has not been tested for immunohistochemistry. I have looked through our catalogue and am very sorry to inform you that we currently do not stock products that suit to your requirement. If you cannot find what you are looking for within the Abcam catalog, I suggest that you click on the link to The World's Antibody Gateway. The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

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