Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (live yeast staining)
Specification
live yeast staining
Preparation
Cell harvesting/tissue preparation method: harvest cells and wash with 1XPBS
Sample buffer: 1XPBS
Sample buffer: 1XPBS
Fixation
no fixation
Permeabilization
No
Gating Strategy
HSV-tag expression
Other product details
Dilution
1/500
Incubation time
20 minute(s) · Temperature: 4°C · Diluent: 1XPBS
Secondary antibody
Name
Non-Abcam antibody was used: PE-conjugated donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/1000
Additional data
Additional Notes
NO staining was observed with anti-HSV antibody. Experiment was precisely controlled: (1). No induction problem (valid yeast, valid reagents) (2). No secondary antibody problem (secondary antibody worked for other items in the same order).
Abcam response
Thank you for submitting an Abreview for this antibody. Flow Cytometry is not an application that is validated for ab9533, therefore we have no information about how well it will work in this application. It is possible that further troubleshooting is necessary to optimize the antibody in this application. Alternatively, it may simply not be suitable for Flow cytometry.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 10 2012