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  1. Link

    hsv1-antibody-ab9533.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus Herpes simplex
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Anti-HSV1 抗体 (ab9533)

  • Datasheet
  • SDS
Reviews (2)Q&A (5)References (24)

Product price, shipping and contact information

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV1 antibody (ab9533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV1 antibody (ab9533)

Key features and details

  • Rabbit polyclonal to HSV1
  • Suitable for: IHC-P
  • Reacts with: Species independent
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Herpes Simplex Virus type 1 (ab124004)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一次抗体
Product image
Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

関連製品

製品の概要

  • 製品名

    Anti-HSV1 antibody
    HSV1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to HSV1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-Pmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Tissue, cells or virus corresponding to HSV1. Whole rabbit corneal cells, infected with herpes simplex virus type I (strain Mac Intyre), were solubilized with detergent.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    ポリクローナル
  • ミエローマ

    unknown
  • アイソタイプ

    IgG
  • 軽鎖の種類

    unknown
  • 研究分野

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Herpes simplex

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Herpes Simplex Virus type 1 (ab124004)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9533の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
Use at an assay dependent concentration.

This antibody may be diluted to a titer of 1:75-1:200 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. Pretreat with citrate buffer pH 6.0.

特記事項
IHC-P
Use at an assay dependent concentration.

This antibody may be diluted to a titer of 1:75-1:200 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. Pretreat with citrate buffer pH 6.0.

ターゲット情報

  • 関連性

    Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low.
  • 別名

    • Herpes simplex virus 1 antibody
    • Herpes simplex virus antibody
    • Herpes simplex virus type 1 antibody
    • HSV 1 antibody
    • Human herpesvirus 1 antibody
    • Human herpesvirus type 1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV1 antibody (ab9533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV1 antibody (ab9533)

    Formalin-fixed, paraffin-embedded HSV1-infected human tissue stained for HSV1 with ab9533 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV1 antibody (ab9533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV1 antibody (ab9533)

    Formalin-fixed, paraffin-embedded HSV1-infected human tissue stained for HSV1 with ab9533 in immunohistochemical analysis.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (24)

ab9533 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9533 は 24 報の論文で使用されています。

  • Liu S  et al. Macrophage polarization contributes to the efficacy of an oncolytic HSV-1 targeting human uveal melanoma in a murine xenograft model. Exp Eye Res 202:108285 (2021). PubMed: 33039456
  • Acuña-Hinrichsen F  et al. Herpes Simplex Virus Type 1 Neuronal Infection Triggers the Disassembly of Key Structural Components of Dendritic Spines. Front Cell Neurosci 15:580717 (2021). PubMed: 33708072
  • He D  et al. TRPC1 participates in the HSV-1 infection process by facilitating viral entry. Sci Adv 6:eaaz3367 (2020). PubMed: 32206724
  • Li W  et al. Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone. Theranostics 10:9032-9049 (2020). PubMed: 32802177
  • Michaelis M  et al. Omeprazole Increases the Efficacy of Acyclovir Against Herpes Simplex Virus Type 1 and 2. Front Microbiol 10:2790 (2019). PubMed: 31849920
View all Publications for this product

レビューと Q&A

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1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HSV1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lip)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0 + .05% Tween-20
Permeabilization
No
Specification
Lip
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Read More

Dr. Kevin Egan

Verified customer

投稿 Mar 23 2018

Flow Cytometry abreview for Anti-HSV1 antibody

Inconclusive
Abreviews
Abreviews
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (live yeast staining)
Specification
live yeast staining
Preparation
Cell harvesting/tissue preparation method: harvest cells and wash with 1XPBS
Sample buffer: 1XPBS
Fixation
no fixation
Permeabilization
No
Gating Strategy
HSV-tag expression
Read More

Abcam user community

Verified customer

投稿 Aug 10 2012

Question

What is the specificity of the antibody ab9533?

Read More

Abcam community

Verified customer

Asked on Mar 20 2013

Answer

I am sorry but the specificity of the antibody has not been studies in detail, I cannot therefore share a great deal of information with you. The antibody has been raised against whole rabbit corneal cells, infected with herpes simplex virus type I. This antibody has been found to react with HSV type 1 specific antigens and with some antigens common for HSV types 1 and 2. The antibody reacts with all the major glycoproteins present in the viral envelope and with at least one core protein as determined by crossed immunoelectrophoresis.

Read More

Abcam Scientific Support

Answered on Mar 20 2013

Question

ab9533 (HSV1 antibody) This antibody contains sodium azide, but what percentage?

Read More

Abcam community

Verified customer

Asked on Jan 31 2007

Answer

Thank you for your patience in this matter. The product, ab9533 (HSV1 antibody), contains 0.05% sodium azide. Please don't hesitate to contact us again if you have further questions.

Read More

Abcam Scientific Support

Answered on Feb 01 2007

Question

What is the concentration?

Read More

Abcam community

Verified customer

Asked on Feb 04 2005

Answer

It is approx 275 ug/ml

Read More

Abcam Scientific Support

Answered on Feb 04 2005

Question

What is the concentraion of Ab in ab9533? The datasheet only suggests a dilution of 1:75 but I would like to know exactly how much protein is in there. Many thanks.

Read More

Abcam community

Verified customer

Asked on Dec 18 2002

Answer

The concentration of this product is 3 mg/ml.

Read More

Abcam Scientific Support

Answered on Dec 20 2002

Question

I am interested in buying ab9533. Has this Ab been tested for Western Blot? What is the optimal titer for WB? I called and found out that this Ab is not in stock. How long will it take for the antibody to arrive to the US?

Read More

Abcam community

Verified customer

Asked on May 30 2002

Answer

This antibody has not been tested in WB, therefore we can't guarantee results. It will take Abcam approximately 7 days to get the antibody, followed by another 24 hours before it arrives in the US.

Read More

Abcam Scientific Support

Answered on May 31 2002

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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