Anti-HSV1 + HSV2 ICP5 Major Capsid Protein 抗体 [3B6] - BSA and Azide free (ab6508)
![Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] - BSA and Azide free (ab6508)](https://www.abcam.co.jp/ps/products/6/ab6508/Images/ab6508-12349-anti-hsv1-hsv2-icp5-major-capsid-protein-antibody-3b6-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] - BSA and Azide free (ab6508)")
Key features and details
- Mouse monoclonal [3B6] to HSV1 + HSV2 ICP5 Major Capsid Protein - BSA and Azide free
- Suitable for: WB, ELISA, ICC/IF
- Isotype: IgG2b
製品の概要
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製品名
Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] - BSA and Azide free -
製品の詳細
Mouse monoclonal [3B6] to HSV1 + HSV2 ICP5 Major Capsid Protein - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISA, ICC/IFmore details -
種交差性
交差種: Other species -
免疫原
Herpes Virus
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
3B6 -
ミエローマ
NS1/1-Ag4-1 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6508の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| ELISA | ||
| ICC/IF |
| 特記事項 |
|---|
追加情報
ICC/IF: Use at an assay dependent dilution (PMID 18701599).
IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200
ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400
Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200
ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400
Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause infections in humans. They are also called Human Herpes Virus 1 and 2 (HHV-1 and HHV-2) and are neurotropic and neuroinvasive viruses; they enter and hide in the human nervous system, accounting for their durability in the human body. -
別名
- Capsid protein VP5 antibody
- Herpes simplex virus 1 major capsid protein antibody
- Herpes simplex virus 2 major capsid protein antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] - BSA and Azide free (ab6508)Image from Ohta A et al., Virol J. 2011 Jul 26;8:365. doi: 10.1186/1743-422X-8-365.; Fig 4.; 26 July 2011, Virology Journal 2011, 8:365Immunofluorescence analysis of HSV infected cells, 7 hours (left) or 17 hours (right) post-infection, staining HSV1 + HSV2 ICP5 Major Capsid Protein with ab6508 at 1/200 dilution.
Cells were fixed for 10 min in paraformaldehyde, permeabilized in 0.1% Triton X-100 and blocked in 4% goat serum, 1% bovine serum albumin, before incubating with primary antibody for 30 min at room temperature. An AlexaFluor®647-conjugated goat anti-mouse IgG1 was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (37)
ab6508 は 37 報の論文で使用されています。
- Wang Y et al. Single-cell RNA-sequencing analysis identifies host long noncoding RNA MAMDC2-AS1 as a co-factor for HSV-1 nuclear transport. Int J Biol Sci 16:1586-1603 (2020). PubMed: 32226304
- Chen Y et al. Single AAV-Mediated CRISPR-SaCas9 Inhibits HSV-1 Replication by Editing ICP4 in Trigeminal Ganglion Neurons. Mol Ther Methods Clin Dev 18:33-43 (2020). PubMed: 32577430
- Wild P et al. Nuclear envelope impairment is facilitated by the herpes simplex virus 1 Us3 kinase. F1000Res 8:198 (2019). PubMed: 31249678
- Sun C et al. Cellular Requirements for Sensing and Elimination of Incoming HSV-1 DNA and Capsids. J Interferon Cytokine Res 39:191-204 (2019). PubMed: 30855198
- Li F et al. Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early Infection. Viruses 11:N/A (2019). PubMed: 31121928