Key features and details
- Rabbit polyclonal to HspBP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の詳細Rabbit polyclonal to HspBP1
特異性This antibody recognises HspBP1 (39kDa) in Western Blots.
アプリケーション適用あり: WB, ICC/IFmore details
Fusion protein corresponding to Human HspBP1.
- This antibody gave a positive signal in HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab3858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
機能Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
配列類似性Contains 4 ARM repeats.
- Information by UniProt
- 1500019G21Rik antibody
- FES1 antibody
- Heat shock protein 70 binding protein antibody
ICC/IF image of ab3858 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab3858, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.
Lane 1 : Anti-HspBP1 antibody (ab3858) at 1/500 dilution
Lane 2 : Anti-HspBP1 antibody (ab3858) at 1/1000 dilution
All lanes : MCF-7 Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39.3 kDa
Observed band size: 39.3 kDa
Exposure time: 2 minutes
Western blot using ab3858 on 20 ug per lane of MCF-7 Whole Cell Lysate. Secondary: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000). Exposure time: 2 mins.
Lane 1: ab3858 at 1/500.
Lane 2: ab3858 at 1/1000.