Anti-HSPA14 抗体 [EPR4210] (ab108612)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4210] to HSPA14
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human, African green monkey
Related conjugates and formulations
製品の概要
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製品名
Anti-HSPA14 antibody [EPR4210]
HSPA14 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4210] to HSPA14 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human, African green monkey
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- K562, COS1, Human fetal liver, and HeLa cell lysates. Human kidney tissue. HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4210 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108612の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/250.
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WB |
1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 55 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity. -
配列類似性
Belongs to the heat shock protein 70 family. -
細胞内局在
Cytoplasm > cytosol. - Information by UniProt
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参照データベース
- Entrez Gene: 51182 Human
- Entrez Gene: 50497 Mouse
- Entrez Gene: 307133 Rat
- Omim: 610369 Human
- SwissProt: Q0VDF9 Human
- SwissProt: Q99M31 Mouse
- SwissProt: Q6AYB4 Rat
- Unigene: 534169 Human
see all -
別名
- Heat shock 70 kDa protein 14 antibody
- Heat shock 70kDa protein 14 antibody
- Heat shock protein HSP60 antibody
see all
画像
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All lanes : Anti-HSPA14 antibody [EPR4210] (ab108612) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : COS-1 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 3 : Human fetal liver tissue lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108612 at 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent staining of HeLa cells using ab108612 at 1/100
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab108612 は 2 報の論文で使用されています。
- Wu IH et al. A role for the ribosome-associated complex in activation of the IRE1 branch of UPR. Cell Rep 35:109217 (2021). PubMed: 34107246
- Morgan SV et al. Proteomic and cellular localisation studies suggest non-tight junction cytoplasmic and nuclear roles for occludin in astrocytes. Eur J Neurosci 47:1444-1456 (2018). PubMed: 29738614