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リコンビナントRabMAb

Anti-Hsp90 抗体 [EPR3953] (ab109248)

Submit a review Submit a question References (7)
Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)
  • Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)
  • Anti-Hsp90 antibody [EPR3953] (ab109248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3953] to Hsp90
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Hsp90 antibody [EPR3953]
    Hsp90 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3953] to Hsp90

  • 由来種

    Rabbit

  • 特異性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Hsp90 alpha and beta recombinant proteins and HeLa and 293T cell lysates IHC-P: Human gastric adenocarcinoma tissue, Human lung carcinoma and Human kidney tissue

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109248の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

  • 配列類似性

    Belongs to the heat shock protein 90 family.

  • ドメイン

    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.

  • 翻訳後修飾

    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.

  • 細胞内局在

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Heat shock 86 kDa antibody
    • Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
    • Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
    • Heat shock protein HSP 90 alpha antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 84 antibody
    • HSP 86 antibody
    • Hsp 90 antibody
    • HSP86 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90AB1 antibody
    • HSP90B antibody
    • HSPC1 antibody
    • HSPC2 antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

画像

  • Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)
    Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)
    All lanes : Anti-Hsp90 antibody [EPR3953] (ab109248) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 3 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Hsp90 with purified ab109248 at 1:50 dilution (19.42 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)

    ab109248, at 1/100, staining Hsp90 in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 antibody [EPR3953] (ab109248)

    ab109248, at 1/100, staining Hsp90 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)
    Western blot - Anti-Hsp90 antibody [EPR3953] (ab109248)
    All lanes : Anti-Hsp90 antibody [EPR3953] (ab109248) at 1/1000 dilution

    Lane 1 : Recombinant Human Hsp90 alpha protein (His tag) (ab48801)
    Lane 2 : Recombinant human Hsp90 beta protein (Active) (ab80033)

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 85 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Hsp90 antibody [EPR3953] (ab109248)
    Anti-Hsp90 antibody [EPR3953] (ab109248)

参考文献 (7)

ab109248 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109248 は 7 報の論文で使用されています。

  • Sha L  et al. Hsp90 inhibitor HSP990 in very low dose upregulates EAAT2 and exerts potent antiepileptic activity. Theranostics 10:8415-8429 (2020). PubMed: 32724478
  • Weigand I  et al. Impact of USP8 Gene Mutations on Protein Deregulation in Cushing Disease. J Clin Endocrinol Metab 104:2535-2546 (2019). PubMed: 30844069
  • Lai R  et al. Role of Hsp90/Akt pathway in the pathogenesis of gentamicin-induced hearing loss. Int J Clin Exp Pathol 11:4431-4438 (2018). PubMed: 31949840
  • Takata T  et al. Generation of glyceraldehyde-derived advanced glycation end-products in pancreatic cancer cells and the potential of tumor promotion. World J Gastroenterol 23:4910-4919 (2017). PubMed: 28785145
  • Sha L  et al. Pharmacologic inhibition of Hsp90 to prevent GLT-1 degradation as an effective therapy for epilepsy. J Exp Med 214:547-563 (2017). PubMed: 28028152
  • Guo W  et al. Targeting GRP75 improves HSP90 inhibitor efficacy by enhancing p53-mediated apoptosis in hepatocellular carcinoma. PLoS One 9:e85766 (2014). IHC ; Human . PubMed: 24465691
  • Guarani V  et al. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol 34:847-61 (2014). PubMed: 24344204

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