Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5382] to Hsp75 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
製品名Anti-Hsp75 antibody [EPR5382] - BSA and Azide free
Hsp75 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5382] to Hsp75 - BSA and Azide free
アプリケーション適用あり: IP, WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P
Synthetic peptide within Human Hsp75 aa 600-700. The exact sequence is proprietary.
Database link: Q12931
ab248218 is the carrier-free version of ab128914 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248218 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).|
機能Chaperone that expresses an ATPase activity.
組織特異性Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
配列類似性Belongs to the heat shock protein 90 family.
- Information by UniProt
- Heat Shock Protein 75 antibody
- Heat shock protein 75 kDa antibody
- Heat shock protein 75 kDa mitochondrial antibody
All lanes : Anti-Hsp75 antibody [EPR5382] (ab128914) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Hela cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 80 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
This data was developed using ab128914, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.