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HSP47 ELISA Kit (ab270882)

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SimpleStep ELISA Protocol Diagram
  • HSP47 standard curve comparison
  • Example of HSP47 standard curve in Sample Diluent NS.
  • Interpolated concentrations of native HSP47 in human HepG2, MCF7, and HeLa extract samples based on a 25 µg/mL extract load.
  • Interpolated concentrations of native HSP47 in human, mouse, and rat liver homogenate extract samples based on a 125 µg/mL extract load.
  • Sandwich ELISA - HSP47 ELISA Kit (ab270882)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 119.23 pg/ml
  • Range: 218.75 pg/ml - 14000 pg/ml
  • Sample type: Cell culture extracts, Cell Lysate, Cit plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    HSP47 ELISA Kit
    Hsp47 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Extract 8 5.9%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Extract 3 8.3%

  • サンプルの種類

    Serum, Cell culture extracts, Cell Lysate, Cit plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    119.23 pg/ml

  • 検出範囲

    218.75 pg/ml - 14000 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 89 88% - 90%
    Cell culture extracts 106 88% - 115%
    Cell Lysate 107 102% - 111%
    Cit plasma 82 80% - 84%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Mouse, Rat, Human

  • 製品の概要

    HSP47 ELISA kit (ab270882) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HSP47 protein in human serum and plasma (citrate) and human, mouse and rat cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate HSP47 with 119.23 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

  • 特記事項

    Heat Shock Protein47 (HSP47) is a collagen specific molecular chaperone protein found in the endoplasmic reticulum. HSP47 assists in the triple-helical formation of collagen and is part of the collagen quality control system. Collagen accounts for approximately 30% of the total protein found in vertebrates and is the major component responsible in providing structural support for a number of tissues. HSP47 can be found constitutively expressed in non-stressed cells associated with collagen expression. Increased HSP47 expression has been associated with increase collagen deposition in arteriosclerosis, myocardial infarction, hepatic fibrosis, renal fibrosis, and pulmonary fibrosis.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
     HSP47  Lyophilized Recombinant Protein 2 vials
    10X HSP47 Capture Antibody 1 x 600µl
    10X HSP47 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BR 1 x 6ml
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • 研究分野

  • 機能

    Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.

  • 関連疾患

    Note=Defects in SERPINH1 may cause severe autosomal recessive osteogenesis imperfecta (OI). Osteogenesis imperfecta defines a group of connective tissue disorders characterized by bone fragility and low bone mass.

  • 配列類似性

    Belongs to the serpin family.

  • 細胞内局在

    Endoplasmic reticulum lumen.
  • Information by UniProt

  • 別名

    • 47 kDa heat shock protein
    • 47 kDa heat shock protein precursor
    • Arsenic transactivated protein 3
    • Arsenic-transactivated protein 3
    • AsTP 3
    • AsTP3
    • CBP 1
    • CBP 2
    • CBP1
    • CBP2
    • Cell proliferation-inducing gene 14 protein
    • Collagen binding protein
    • Collagen binding protein 1
    • Collagen binding protein 2
    • Collagen-binding protein
    • Collagen-binding protein 2
    • Colligen
    • Colligin
    • Colligin 1
    • Colligin 2
    • colligin-1
    • colligin-2
    • gp46
    • Heat shock protein 47
    • Heat-shock protein 47
    • HGNC 1547
    • Hsp 47
    • J6
    • OI10
    • PIG 14
    • PIG14
    • PPROM
    • Proliferation inducing gene 14
    • Proliferation inducing gene 14 protein
    • RA A47
    • RA-A47
    • Rheumatoid arthritis antigen A 47
    • rheumatoid arthritis antigen A-47
    • Rheumatoid arthritis related antigen RA A47
    • Rheumatoid arthritis-related antigen RA-A47
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
    • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2)
    • Serine or cysteine proteinase inhibitor clade H member 1
    • Serine or cysteine proteinase inhibitor clade H member 2
    • SERPH_HUMAN
    • Serpin H1
    • Serpin peptidase inhibitor clade H member 1
    • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
    • Serpin peptidase inhibitor, clade H, member 1
    • SERPINH1
    • SERPINH2
    see all

  • 参照データベース

    see all

関連製品

画像

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • HSP47 standard curve comparison
    HSP47 standard curve comparison

    Standard Curve comparison between the original HSP47 SimpleStep ELISA kit (ab202407) and current HSP47 SimpleStep ELISA kit (ab270882).

  • Example of HSP47 standard curve in Sample Diluent NS.
    Example of HSP47 standard curve in Sample Diluent NS.

    The HSP47 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native HSP47 in human HepG2, MCF7, and HeLa extract samples based on a 25 µg/mL extract load.
    Interpolated concentrations of native HSP47 in human HepG2, MCF7, and HeLa extract samples based on a 25 µg/mL extract load.

    The concentrations of HSP47 were measured in duplicate and interpolated from the HSP47 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HSP47 concentration was determined to be 14.52 ng/mL in HepG2, 14.19 ng/ml in MCF7, and 12.46 ng/mL in HeLa extract samples.

  • Interpolated concentrations of native HSP47 in human, mouse, and rat liver homogenate extract samples based on a 125 µg/mL extract load.
    Interpolated concentrations of native HSP47 in human, mouse, and rat liver homogenate extract samples based on a 125 µg/mL extract load.

    The concentrations of HSP47 were measured in duplicate and interpolated from the HSP47 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HSP47 concentration was determined to be 2.16 ng/mL in human, 1.97 ng/ml in mouse, and 8.90 ng/mL in rat liver homogenate extract samples.

  • Sandwich ELISA - HSP47 ELISA Kit (ab270882)
    Sandwich ELISA - HSP47 ELISA Kit (ab270882)
    To learn more about the advantages of recombinant antibodies see here.

参考文献 (0)

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