Application
Western blot
Sample
Human Cell lysate - whole cell (colon)
Loading amount
20 µg
Specification
colon
Treatment
heat shock
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: JacksonImmunolab
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 75 kDa Non-specific: 150, 100, 70, 65 kDa
Positive control
colon cancer cell sample treated with heat shock
Negative control
colon cancer cell sample with no heat shock
Additional data
Additional Notes
Membrane type :PVDF
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Young Ju Yoon
Verified customer
投稿 Sep 04 2009