Anti-HSF1 (phospho S326) 抗体 [EP1713Y] (ab76076)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1713Y] to HSF1 (phospho S326)
- Suitable for: Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HSF1 (phospho S326) antibody [EP1713Y]
HSF1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1713Y] to HSF1 (phospho S326) -
由来種
Rabbit -
アプリケーション
適用あり: Dot blot, WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa cell lysates and HeLa cell lysate treated with heat (44ºC)
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1713Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76076の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Dot blot |
Use a concentration of 0.338 µg/ml.
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WB | (1) |
1/5000 - 1/10000. Predicted molecular weight: 57 kDa.
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特記事項 |
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Dot blot
Use a concentration of 0.338 µg/ml. |
WB
1/5000 - 1/10000. Predicted molecular weight: 57 kDa. |
ターゲット情報
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機能
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. -
配列類似性
Belongs to the HSF family. -
ドメイン
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
翻訳後修飾
Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules. - Information by UniProt
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参照データベース
- Entrez Gene: 3297 Human
- Omim: 140580 Human
- SwissProt: Q00613 Human
- Unigene: 530227 Human
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別名
- Heat shock factor 1 antibody
- Heat shock factor protein 1 antibody
- Heat shock transcription factor 1 antibody
see all
画像
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All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 0.169 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with heat at 43? for 30 minutes whole cell lysate
Lane 3 : HeLa treated with heat at 43? for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.02 µg/ml
Predicted band size: 57 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
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All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat (42 ?) whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with heat (42 ?) whole cell lysates. Then the membrane was incubated with phosphatase
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted? -
Dot blot analysis of HSF1 (phospho S326) peptide (Lane 1), HSF1 non-phospho peptide (Lane 2) labelling HSF1 (phospho S326) with purified ab76076 at 0.338 ug/mL. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at 0.01 ug/mL.
Blocking and dilution buffer: 5% NFDM/TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (48)
ab76076 は 48 報の論文で使用されています。
- Garofalo M et al. RNA Molecular Signature Profiling in PBMCs of Sporadic ALS Patients: HSP70 Overexpression Is Associated with Nuclear SOD1. Cells 11:N/A (2022). PubMed: 35053410
- Wang M et al. The Therapeutic Roles of Recombinant Hsp90α on Cornea Epithelial Injury. Invest Ophthalmol Vis Sci 63:30 (2022). PubMed: 35201262
- Steurer C et al. Quantitative Comparison of HSF1 Activators. Mol Biotechnol 64:873-887 (2022). PubMed: 35218516
- Iuliano L et al. Proteotoxic stress-induced apoptosis in cancer cells: understanding the susceptibility and enhancing the potency. Cell Death Discov 8:407 (2022). PubMed: 36195608
- Hoff FW et al. Heat shock factor 1 (HSF1-pSer326) predicts response to bortezomib-containing chemotherapy in pediatric AML: a COG report. Blood 137:1050-1060 (2021). PubMed: 32959058